Human ISL1 ELISA Kit

Alternative name
Human islet-1 ELISA Kit;Human ISLET1 ELISA Kit;Human Isl-1 ELISA Kit;Human ISL LIM homeobox 1 ELISA Kit;Human insulin gene enhancer protein ISL-1 ELISA Kit;Human ISL1 transcription factor, LIM/homeodomain ELISA Kit;
Catalog
E021090
species
Human
GeneISL1
SpecificityThis assay has high sensitivity and excellent specificity for detection of ISL1. No significant cross-reactivity or interference between ISL1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ISL1 is typically less than 0.063ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman ISL1 ELISA Kit allows for the in vitro quantitative determination of ISL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ISL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ISL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ISL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ISL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Insulin gene enhancer protein ISL-1 Protein information
Uniprot ID ISL1_HUMAN
Uniprot AC P61371; P20663; P47894;
UniGene Hs.505;
GeneID 3670
KEGG hsa:3670;
Human Insulin gene enhancer protein ISL-1 Protein SEQUENCE
SEQUENCE 349 AA; 39036 MW; 4DB82FD09154F404 CRC64;
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ
YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY
HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL
HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY
AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ
PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
UCSC uc003jor.4; human.;

Category

Human ISL1 ELISA Kit

Human islet-1 ELISA Kit;Human ISLET1 ELISA Kit;Human Isl-1 ELISA Kit;Human ISL LIM homeobox 1 ELISA Kit;Human insulin gene enhancer protein ISL-1 ELISA Kit;Human ISL1 transcription factor, LIM/homeodomain ELISA Kit;