Human IARS ELISA Kit

Alternative name
Human isoleucyl-tRNA synthetase ELISA Kit;Human IRS ELISA Kit;Human ILERS ELISA Kit;Human GRIDHH ELISA Kit;Human IARS1 ELISA Kit;Human ILRS ELISA Kit;Human PRO0785 ELISA Kit;Human isoleucine--tRNA ligase, cytoplasmic ELISA Kit;Human isoleucine tRNA ligase 1, cytoplasmic ELISA Kit;
Catalog
E021133
species
Human
GeneIARS
SpecificityThis assay has high sensitivity and excellent specificity for detection of Isoleucyl tRNA Synthetase (IARS). No significant cross-reactivity or interference between Isoleucyl tRNA Synthetase (IARS) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.108ng/mL.
Intended UseHuman IARS ELISA Kit allows for the in vitro quantitative determination of IARS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isoleucyl tRNA Synthetase (IARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isoleucyl tRNA Synthetase (IARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isoleucyl tRNA Synthetase (IARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isoleucyl tRNA Synthetase (IARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Isoleucine--tRNA ligase, cytoplasmic Protein information
Uniprot ID SYIC_HUMAN
Uniprot AC P41252; A8KAE9; Q5TCD0; Q7Z3T4; Q9H588;
UniGene Hs.445403;
GeneID 3376
KEGG hsa:3376;
Human Isoleucine--tRNA ligase, cytoplasmic Protein SEQUENCE
SEQUENCE 1262 AA; 144498 MW; 73CB3967A6868005 CRC64;
MLQQVPENIN FPAEEEKILE FWTEFNCFQE CLKQSKHKPK FTFYDGPPFA
TGLPHYGHIL AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL
GIRGPEDVAK MGITEYNNQC RAIVMRYSAE WKSTVSRLGR WIDFDNDYKT
LYPQFMESVW WVFKQLYDKG LVYRGVKVMP FSTACNTPLS NFESHQNYKD
VQDPSVFVTF PLEEDETVSL VAWTTTPWTL PSNLAVCVNP EMQYVKIKDV
ARGRLLILME ARLSALYKLE SDYEILERFP GAYLKGKKYR PLFDYFLKCK
ENGAFTVLVD NYVKEEEGTG VVHQAPYFGA EDYRVCMDFN IIRKDSLPVC
PVDASGCFTT EVTDFAGQYV KDADKSIIRT LKEQGRLLVA TTFTHSYPFC
WRSDTPLIYK AVPSWFVRVE NMVDQLLRNN DLCYWVPELV REKRFGNWLK
DARDWTISRN RYWGTPIPLW VSDDFEEVVC IGSVAELEEL SGAKISDLHR
ESVDHLTIPS RCGKGSLHRI SEVFDCWFES GSMPYAQVHY PFENKREFED
AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLVLASDGQK
MSKRKKNYPD PVSIIQKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK
DVLLPWYNAY RFLIQNVLRL QKEEEIEFLY NENTVRESPN ITDRWILSFM
QSLIGFFETE MAAYRLYTVV PRLVKFVDIL TNWYVRMNRR RLKGENGMED
CVMALETLFS VLLSLCRLMA PYTPFLTELM YQNLKVLIDP VSVQDKDTLS
IHYLMLPRVR EELIDKKTES AVSQMQSVIE LGRVIRDRKT IPIKYPLKEI
VVIHQDPEAL KDIKSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV
LGKRLKGAFK AVMTSIKQLS SEELEQFQKT GTIVVEGHEL HDEDIRLMYT
FDQATGGTAQ FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK
CNLVPTDEIT VYYKAKSEGT YLNSVIESHT EFIFTTIKAP LKPYPVSPSD
KVLIQEKTQL KGSELEITLT RGSSLPGPAC AYVNLNICAN GSEQGGVLLL
ENPKGDNRLD LLKLKSVVTS IFGVKNTELA VFHDETEIQN QTDLLSLSGK
TLCVTAGSAP SLINSSSTLL CQYINLQLLN AKPQECLMGT VGTLLLENPL
GQNGLTHQGL LYEAAKVFGL RSRKLKLFLN ETQTQEITED IPVKTLNMKT
VYVSVLPTTA DF
UCSC uc004art.4; human.;

Category

Human IARS ELISA Kit

Human isoleucyl-tRNA synthetase ELISA Kit;Human IRS ELISA Kit;Human ILERS ELISA Kit;Human GRIDHH ELISA Kit;Human IARS1 ELISA Kit;Human ILRS ELISA Kit;Human PRO0785 ELISA Kit;Human isoleucine--tRNA ligase, cytoplasmic ELISA Kit;Human isoleucine tRNA ligase 1, cytoplasmic ELISA Kit;