Human IZUMO1 ELISA Kit

Alternative name
Human oocyte binding/fusion factor ELISA Kit;Human OBF ELISA Kit;Human sperm-specific protein Izumo ELISA Kit;Human IZUMO ELISA Kit;Human izumo sperm-egg fusion 1 ELISA Kit;Human izumo sperm-egg fusion protein 1 ELISA Kit;
Catalog
E021149
species
Human
GeneIZUMO1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 9.4pg/ml
Intended UseHuman IZUMO1 ELISA Kit allows for the in vitro quantitative determination of IZUMO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IZUMO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IZUMO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IZUMO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IZUMO1 can be calculated.
Human Izumo sperm-egg fusion protein 1 Protein information
Uniprot ID IZUM1_HUMAN
Uniprot AC Q8IYV9; Q6Q8P6; Q6Q8P7;
UniGene Hs.400688;
GeneID 284359
KEGG hsa:284359;
Human Izumo sperm-egg fusion protein 1 Protein SEQUENCE
SEQUENCE 350 AA; 38930 MW; 8741BB3F93ABCFA9 CRC64;
MGPHFTLLCA ALAGCLLPAE GCVICDPSVV LALKSLEKDY LPGHLDAKHH
KAMMERVENA VKDFQELSLN EDAYMGVVDE ATLQKGSWSL LKDLKRITDS
DVKGDLFVKE LFWMLHLQKE TFATYVARFQ KEAYCPNKCG VMLQTLIWCK
NCKKEVHACR KSYDCGERNV EVPQMEDMIL DCELNWHQAS EGLTDYSFYR
VWGNNTETLV SKGKEATLTK PMVGPEDAGS YRCELGSVNS SPATIINFHV
TVLPKMIKEE KPSPNIVTPG EATTESSISL QPLQPEKMLA SRLLGLLICG
SLALITGLTF AIFRRRKVID FIKSSLFGLG SGAAEQTQVP KEKATDSRQQ
UCSC uc002pkj.3; human. [Q8IYV9-1];

Category

Human IZUMO1 ELISA Kit

Human oocyte binding/fusion factor ELISA Kit;Human OBF ELISA Kit;Human sperm-specific protein Izumo ELISA Kit;Human IZUMO ELISA Kit;Human izumo sperm-egg fusion 1 ELISA Kit;Human izumo sperm-egg fusion protein 1 ELISA Kit;