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Human KPNB1 ELISA Kit

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  • Alternative name

    Human Importin-90 ELISA Kit;Human Karyopherin subunit beta-1 ELISA Kit;Human nuclear factor p97 ELISA Kit;Human pore targeting complex 97 kDa subunit ELISA Kit;Human PTAC97 ELISA Kit;Human NTF97 ELISA Kit;Human IMB1 ELISA Kit;Human IPO1 ELISA Kit;Human IPOB ELISA Kit;Human Impnb ELISA Kit;Human karyopherin subunit beta 1 ELISA Kit;Human importin subunit beta-1 ELISA Kit;Human importin 1 ELISA Kit;Human importin 90 ELISA Kit;Human importin beta-1 subunit ELISA Kit;Human karyopherin (importin) beta 1 ELISA Kit;
  • Catalog
    E021356
  • species
    Human
  • GeneKPNB1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Karyopherin Beta (KPNb1). No significant cross-reactivity or interference between Karyopherin Beta (KPNb1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.28ng/mL.
  • Intended UseHuman KPNB1 ELISA Kit allows for the in vitro quantitative determination of KPNB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Beta (KPNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Karyopherin Beta (KPNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Beta (KPNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Beta (KPNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Importin subunit beta-1 Protein information
  • Uniprot ID IMB1_HUMAN
  • Uniprot AC Q14974; B7ZAV6; D3DTT3; Q14637; Q53XN2; Q96J27;
  • UniGene Hs.532793;
  • GeneID 3837
  • KEGG hsa:3837;
  • Human Importin subunit beta-1 Protein SEQUENCE

  • SEQUENCE 876 AA; 97170 MW; F3BB8B73E7E51639 CRC64;

    MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS

    QVARVAAGLQ IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG

    TETYRPSSAS QCVAGIACAE IPVNQWPELI PQLVANVTNP NSTEHMKEST

    LEAIGYICQD IDPEQLQDKS NEILTAIIQG MRKEEPSNNV KLAATNALLN

    SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN LVKIMSLYYQ

    YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA

    AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV

    CLMLLATCCE DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GCILEGPEPS

    QLKPLVIQAM PTLIELMKDP SVVVRDTAAW TVGRICELLP EAAINDVYLA

    PLLQCLIEGL SAEPRVASNV CWAFSSLAEA AYEAADVADD QEEPATYCLS

    SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA KDCYPAVQKT

    TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA

    LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF

    KPFLGIGLKN YAEYQVCLAA VGLVGDLCRA LQSNIIPFCD EVMQLLLENL

    GNENVHRSVK PQILSVFGDI ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS

    DYDMVDYLNE LRESCLEAYT GIVQGLKGDQ ENVHPDVMLV QPRVEFILSF

    IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR PMIHELLTEG

    RRSKTNKAKT LATWATKELR KLKNQA

  • UCSC uc002ilt.3; human. [Q14974-1];



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