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Alternative name
Kelch-like protein 41 ELISA KIT; Kel-like protein 23 ELISA KIT; Kelch repeat and BTB domain-containing protein 10 ELISA KIT; Kelch-related protein 1 ELISA KIT; SarcosinKLHL41 ELISA KIT; KBTBD10 ELISA KIT; KRP1 ELISA KIT
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Catalog
E021385
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species
Human
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GeneKBTBD10
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human KBTBD10. No significant cross-reactivity or interference between human KBTBD10 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human KBTBD10 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
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Intended UseHuman KBTBD10 ELISA Kit allows for the in vitro quantitative determination of KBTBD10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KBTBD10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KBTBD10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KBTBD10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KBTBD10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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