Human KRT13 ELISA Kit

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  • Alternative name

    Human Cytokeratin-13 ELISA Kit;Human CK-13 ELISA Kit;Human Keratin-13 ELISA Kit;Human K13 ELISA Kit;Human CK13 ELISA Kit;Human WSN2 ELISA Kit;Human keratin 13 ELISA Kit;Human keratin, type I cytoskeletal 13 ELISA Kit;Human cytokeratin 13 ELISA Kit;Human keratin 13, type I ELISA Kit;

  • Catalog
    E021440
  • species
    Human
  • GeneKRT13
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 13 (KRT13). No significant cross-reactivity or interference between Keratin 13 (KRT13) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.1pg/mL.
  • Intended UseHuman KRT13 ELISA Kit allows for the in vitro quantitative determination of KRT13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 13 (KRT13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 13 (KRT13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 13 (KRT13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 13 (KRT13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Keratin, type I cytoskeletal 13 Protein information
  • Uniprot ID K1C13_HUMAN
  • Uniprot AC P13646; Q53G54; Q6AZK5; Q8N240;
  • UniGene Hs.654550;
  • GeneID 3860
  • KEGG hsa:3860;
  • Human Keratin, type I cytoskeletal 13 Protein SEQUENCE
  • SEQUENCE 458 AA; 49588 MW; A51AECFD6195ACB4 CRC64;

    MSLRLQSSSA SYGGGFGGGS CQLGGGRGVS TCSTRFVSGG SAGGYGGGVS

    CGFGGGAGSG FGGGYGGGLG GGYGGGLGGG FGGGFAGGFV DFGACDGGLL

    TGNEKITMQN LNDRLASYLE KVRALEEANA DLEVKIRDWH LKQSPASPER

    DYSPYYKTIE ELRDKILTAT IENNRVILEI DNARLAADDF RLKYENELAL

    RQSVEADING LRRVLDELTL SKTDLEMQIE SLNEELAYMK KNHEEEMKEF

    SNQVVGQVNV EMDATPGIDL TRVLAEMREQ YEAMAERNRR DAEEWFHTKS

    AELNKEVSTN TAMIQTSKTE ITELRRTLQG LEIELQSQLS MKAGLENTVA

    ETECRYALQL QQIQGLISSI EAQLSELRSE MECQNQEYKM LLDIKTRLEQ

    EIATYRSLLE GQDAKMIGFP SSAGSVSPRS TSVTTTSSAS VTTTSNASGR

    RTSDVRRP

  • UCSC uc002hwu.2; human. [P13646-1];



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