Human KRT14 ELISA Kit

Alternative name
Human Cytokeratin-14 ELISA Kit;Human CK-14 ELISA Kit;Human Keratin-14 ELISA Kit;Human K14 ELISA Kit;Human CK14 ELISA Kit;Human EBS3 ELISA Kit;Human EBS4 ELISA Kit;Human NFJ ELISA Kit;Human keratin 14 ELISA Kit;Human keratin, type I cytoskeletal 14 ELISA Kit;Human cytokeratin 14 ELISA Kit;Human keratin 14, type I ELISA Kit;
Catalog
E021441
species
Human
GeneKRT14
SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 14 (KRT14). No significant cross-reactivity or interference between Keratin 14 (KRT14) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman KRT14 ELISA Kit allows for the in vitro quantitative determination of KRT14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 14 (KRT14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 14 (KRT14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 14 (KRT14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 14 (KRT14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Keratin, type I cytoskeletal 14 Protein information
Uniprot ID K1C14_HUMAN
Uniprot AC P02533; Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4;
UniGene Hs.654380;
GeneID 3861
KEGG hsa:3861;
Human Keratin, type I cytoskeletal 14 Protein SEQUENCE
SEQUENCE 472 AA; 51561 MW; 120BA30BA2F8E397 CRC64;
MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL
SVSSSRFSSG GACGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGAGLGGGF
GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW
YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF
RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK
KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK
DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS
MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI
LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT
KVMDVHDGKV VSTHEQVLRT KN
UCSC uc002hxf.3; human.;

Category

Human KRT14 ELISA Kit