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Human KRT19 ELISA Kit

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  • Alternative name

    Human Cytokeratin-19 ELISA Kit;Human CK-19 ELISA Kit;Human Keratin-19 ELISA Kit;Human K19 ELISA Kit;Human CK19 ELISA Kit;Human K1CS ELISA Kit;Human keratin 19 ELISA Kit;Human keratin, type I cytoskeletal 19 ELISA Kit;Human 40-kDa keratin intermediate filament ELISA Kit;Human cytokeratin 19 ELISA Kit;Human keratin 19, type I ELISA Kit;Human keratin, type I, 40-kd ELISA Kit;
  • Catalog
    E021452
  • species
    Human
  • GeneKRT19
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 19 (KRT19). No significant cross-reactivity or interference between Keratin 19 (KRT19) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 4.9pg/mL.
  • Intended UseHuman KRT19 ELISA Kit allows for the in vitro quantitative determination of KRT19 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 19 (KRT19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 19 (KRT19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 19 (KRT19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 19 (KRT19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Keratin, type I cytoskeletal 19 Protein information
  • Uniprot ID K1C19_HUMAN
  • Uniprot AC Q9P1Y4;
  • UniGene Hs.654568;
  • GeneID 3880
  • KEGG hsa:3880;
  • Human Keratin, type I cytoskeletal 19 Protein SEQUENCE

  • SEQUENCE 400 AA; 44106 MW; C559BBBACCE32DCB CRC64;

    MTSYSYRQSS ATSSFGGLGG GSVRFGPGVA FRAPSIHGGS GGRGVSVSSA

    RFVSSSSSGA YGGGYGGVLT ASDGLLAGNE KLTMQNLNDR LASYLDKVRA

    LEAANGELEV KIRDWYQKQG PGPSRDYSHY YTTIQDLRDK ILGATIENSR

    IVLQIDNARL AADDFRTKFE TEQALRMSVE ADINGLRRVL DELTLARTDL

    EMQIEGLKEE LAYLKKNHEE EISTLRGQVG GQVSVEVDSA PGTDLAKILS

    DMRSQYEVMA EQNRKDAEAW FTSRTEELNR EVAGHTEQLQ MSRSEVTDLR

    RTLQGLEIEL QSQLSMKAAL EDTLAETEAR FGAQLAHIQA LISGIEAQLG

    DVRADSERQN QEYQRLMDIK SRLEQEIATY RSLLEGQEDH YNNLSASKVL

  • UCSC uc002hxd.5; human.;



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