Human KRT2 ELISA Kit

Alternative name
Human cytokeratin-2e ELISA Kit;Human CK-2e ELISA Kit;Human epithelial keratin-2e ELISA Kit;Human keratin-2 epidermis ELISA Kit;Human keratin-2e ELISA Kit;Human K2e ELISA Kit;Human type-II keratin Kb2 ELISA Kit;Human KRT2A ELISA Kit;Human KRT2E ELISA Kit;Human KRTE ELISA Kit;Human keratin 2 ELISA Kit;Human keratin, type II cytoskeletal 2 epidermal ELISA Kit;Human keratin 2, type II ELISA Kit;
Catalog
E021454
species
Human
GeneKRT2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 2 (KRT2). No significant cross-reactivity or interference between Keratin 2 (KRT2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman KRT2 ELISA Kit allows for the in vitro quantitative determination of KRT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 2 (KRT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 2 (KRT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 2 (KRT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 2 (KRT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Keratin, type II cytoskeletal 2 epidermal Protein information
Uniprot ID K22E_HUMAN
Uniprot AC P35908; Q4VAQ2;
UniGene Hs.707;
GeneID 3849
KEGG hsa:3849;
Human Keratin, type II cytoskeletal 2 epidermal Protein SEQUENCE
SEQUENCE 639 AA; 65433 MW; B80526BAF70078A7 CRC64;
MSCQISCKSR GRGGGGGGFR GFSSGSAVVS GGSRRSTSSF SCLSRHGGGG
GGFGGGGFGS RSLVGLGGTK SISISVAGGG GGFGAAGGFG GRGGGFGGGS
SFGGGSGFSG GGFGGGGFGG GRFGGFGGPG GVGGLGGPGG FGPGGYPGGI
HEVSVNQSLL QPLNVKVDPE IQNVKAQERE QIKTLNNKFA SFIDKVRFLE
QQNQVLQTKW ELLQQMNVGT RPINLEPIFQ GYIDSLKRYL DGLTAERTSQ
NSELNNMQDL VEDYKKKYED EINKRTAAEN DFVTLKKDVD NAYMIKVELQ
SKVDLLNQEI EFLKVLYDAE ISQIHQSVTD TNVILSMDNS RNLDLDSIIA
EVKAQYEEIA QRSKEEAEAL YHSKYEELQV TVGRHGDSLK EIKIEISELN
RVIQRLQGEI AHVKKQCKNV QDAIADAEQR GEHALKDARN KLNDLEEALQ
QAKEDLARLL RDYQELMNVK LALDVEIATY RKLLEGEECR MSGDLSSNVT
VSVTSSTISS NVASKAAFGG SGGRGSSSGG GYSSGSSSYG SGGRQSGSRG
GSGGGGSISG GGYGSGGGSG GRYGSGGGSK GGSISGGGYG SGGGKHSSGG
GSRGGSSSGG GYGSGGGGSS SVKGSSGEAF GSSVTFSFR
UCSC uc001sat.4; human.;

Category

Human KRT2 ELISA Kit