Human KRT8 ELISA Kit

Alternative name
Human cytokeratin-8 ELISA Kit;Human CK-8 ELISA Kit;Human Keratin-8 ELISA Kit;Human K8 ELISA Kit;Human type-II keratin Kb8 ELISA Kit;Human CYK8 ELISA Kit;Human CARD2 ELISA Kit;Human CK8 ELISA Kit;Human K2C8 ELISA Kit;Human KO ELISA Kit;Human keratin 8 ELISA Kit;Human keratin, type II cytoskeletal 8 ELISA Kit;Human keratin 8, type II ELISA Kit;
Catalog
E021498
species
Human
GeneKRT8
SpecificityThis assay has high sensitivity and excellent specificity for detection of human KRT8. No significant cross-reactivity or interference between human KRT8 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human KRT8 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman KRT8 ELISA Kit allows for the in vitro quantitative determination of KRT8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRT8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Keratin, type II cytoskeletal 8 Protein information
Uniprot ID K2C8_HUMAN
Uniprot AC Q6DHW5; Q6GMY0; Q6P4C7; Q96J60;
UniGene Hs.533782; Hs.708445;
GeneID 3856
KEGG hsa:3856;
Human Keratin, type II cytoskeletal 8 Protein SEQUENCE
SEQUENCE 483 AA; 53704 MW; B0BC730B65929D37 CRC64;
MSIRVTQKSY KVSTSGPRAF SSRSYTSGPG SRISSSSFSR VGSSNFRGGL
GGGYGGASGM GGITAVTVNQ SLLSPLVLEV DPNIQAVRTQ EKEQIKTLNN
KFASFIDKVR FLEQQNKMLE TKWSLLQQQK TARSNMDNMF ESYINNLRRQ
LETLGQEKLK LEAELGNMQG LVEDFKNKYE DEINKRTEME NEFVLIKKDV
DEAYMNKVEL ESRLEGLTDE INFLRQLYEE EIRELQSQIS DTSVVLSMDN
SRSLDMDSII AEVKAQYEDI ANRSRAEAES MYQIKYEELQ SLAGKHGDDL
RRTKTEISEM NRNISRLQAE IEGLKGQRAS LEAAIADAEQ RGELAIKDAN
AKLSELEAAL QRAKQDMARQ LREYQELMNV KLALDIEIAT YRKLLEGEES
RLESGMQNMS IHTKTTSGYA GGLSSAYGGL TSPGLSYSLG SSFGSGAGSS
SFSRTSSSRA VVVKKIETRD GKLVSESSDV LPK
UCSC uc001sbd.3; human. [P05787-1];

Category

Human KRT8 ELISA Kit