Human KERA ELISA Kit

Alternative name
Human KTN ELISA Kit;Human keratan sulfate proteoglycan keratocan ELISA Kit;Human SLRR2B ELISA Kit;Human CNA2 ELISA Kit;Human keratocan ELISA Kit;
Catalog
E021624
species
Human
GeneKERA
SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratocan (KERA). No significant cross-reactivity or interference between Keratocan (KERA) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.127ng/mL.
Intended UseHuman KERA ELISA Kit allows for the in vitro quantitative determination of KERA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratocan (KERA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratocan (KERA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratocan (KERA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratocan (KERA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Keratocan Protein information
Uniprot ID KERA_HUMAN
Uniprot AC O60938;
UniGene Hs.125750;
GeneID 11081
KEGG hsa:11081;
Human Keratocan Protein SEQUENCE
SEQUENCE 352 AA; 40509 MW; 0CF8DEC938852D28 CRC64;
MAGTICFIMW VLFITDTVWS RSVRQVYEVH DSDDWTIHDF ECPMECFCPP
SFPTALYCEN RGLKEIPAIP SRIWYLYLQN NLIETIPEKP FENATQLRWI
NLNKNKITNY GIEKGALSQL KKLLFLFLED NELEEVPSPL PRSLEQLQLA
RNKVSRIPQG TFSNLENLTL LDLQNNKLVD NAFQRDTFKG LKNLMQLNMA
KNALRNMPPR LPANTMQLFL DNNSIEGIPE NYFNVIPKVA FLRLNHNKLS
DEGLPSRGFD VSSILDLQLS HNQLTKVPRI SAHLQHLHLD HNKIKSVNVS
VICPSPSMLP AERDSFSYGP HLRYLRLDGN EIKPPIPMAL MTCFRLLQAV
II
UCSC uc001tbl.4; human.;

Category

Human KERA ELISA Kit

Human KTN ELISA Kit;Human keratan sulfate proteoglycan keratocan ELISA Kit;Human SLRR2B ELISA Kit;Human CNA2 ELISA Kit;Human keratocan ELISA Kit;