Human KIF5A ELISA Kit

Alternative name
Human kinesin heavy chain neuron-specific 1 ELISA Kit;Human neuronal kinesin heavy chain ELISA Kit;Human NKHC ELISA Kit;Human NKHC1 ELISA Kit;Human D12S1889 ELISA Kit;Human MY050 ELISA Kit;Human NEIMY ELISA Kit;Human SPG10 ELISA Kit;Human kinesin family member 5A ELISA Kit;Human kinesin heavy chain isoform 5A ELISA Kit;Human KIF5A variant protein ELISA Kit;Human kinesin, heavy chain, neuron-specific ELISA Kit;
Catalog
E021864
species
Human
GeneKIF5A
SpecificityThis assay has high sensitivity and excellent specificity for detection of Kinesin Family, Member 5A (KIF5A). No significant cross-reactivity or interference between Kinesin Family, Member 5A (KIF5A) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.118ng/mL.
Intended UseHuman KIF5A ELISA Kit allows for the in vitro quantitative determination of KIF5A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 5A (KIF5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin Family, Member 5A (KIF5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 5A (KIF5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin Family, Member 5A (KIF5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Kinesin heavy chain isoform 5A Protein information
Uniprot ID KIF5A_HUMAN
Uniprot AC Q12840; A6H8M5; Q4LE26;
UniGene Hs.151219;
GeneID 3798
KEGG hsa:3798;
Human Kinesin heavy chain isoform 5A Protein SEQUENCE
SEQUENCE 1032 AA; 117378 MW; C4C6C12342040796 CRC64;
MAETNNECSI KVLCRFRPLN QAEILRGDKF IPIFQGDDSV VIGGKPYVFD
RVFPPNTTQE QVYHACAMQI VKDVLAGYNG TIFAYGQTSS GKTHTMEGKL
HDPQLMGIIP RIARDIFNHI YSMDENLEFH IKVSYFEIYL DKIRDLLDVT
KTNLSVHEDK NRVPFVKGCT ERFVSSPEEI LDVIDEGKSN RHVAVTNMNE
HSSRSHSIFL INIKQENMET EQKLSGKLYL VDLAGSEKVS KTGAEGAVLD
EAKNINKSLS ALGNVISALA EGTKSYVPYR DSKMTRILQD SLGGNCRTTM
FICCSPSSYN DAETKSTLMF GQRAKTIKNT ASVNLELTAE QWKKKYEKEK
EKTKAQKETI AKLEAELSRW RNGENVPETE RLAGEEAALG AELCEETPVN
DNSSIVVRIA PEERQKYEEE IRRLYKQLDD KDDEINQQSQ LIEKLKQQML
DQEELLVSTR GDNEKVQREL SHLQSENDAA KDEVKEVLQA LEELAVNYDQ
KSQEVEEKSQ QNQLLVDELS QKVATMLSLE SELQRLQEVS GHQRKRIAEV
LNGLMKDLSE FSVIVGNGEI KLPVEISGAI EEEFTVARLY ISKIKSEVKS
VVKRCRQLEN LQVECHRKME VTGRELSSCQ LLISQHEAKI RSLTEYMQSV
ELKKRHLEES YDSLSDELAK LQAQETVHEV ALKDKEPDTQ DADEVKKALE
LQMESHREAH HRQLARLRDE INEKQKTIDE LKDLNQKLQL ELEKLQADYE
KLKSEEHEKS TKLQELTFLY ERHEQSKQDL KGLEETVARE LQTLHNLRKL
FVQDVTTRVK KSAEMEPEDS GGIHSQKQKI SFLENNLEQL TKVHKQLVRD
NADLRCELPK LEKRLRATAE RVKALEGALK EAKEGAMKDK RRYQQEVDRI
KEAVRYKSSG KRGHSAQIAK PVRPGHYPAS SPTNPYGTRS PECISYTNSL
FQNYQNLYLQ ATPSSTSDMY FANSCTSSGA TSSGGPLASY QKANMDNGNA
TDINDNRSDL PCGYEAEDQA KLFPLHQETA AS
UCSC uc001sor.2; human.;

Category

Human KIF5A ELISA Kit