Human KRAS ELISA Kit

Alternative name
Human K-Ras 2 ELISA Kit;Human KI-RAS ELISA Kit;Human C-K-RAS ELISA Kit;Human c-Ki-ras ELISA Kit;Human KRAS2 ELISA Kit;Human RASK2 ELISA Kit;Human CFC2 ELISA Kit;Human K-RAS2A ELISA Kit;Human K-RAS2B ELISA Kit;Human K-RAS4A ELISA Kit;Human K-RAS4B ELISA Kit;Human KRAS1 ELISA Kit;Human NS ELISA Kit;Human NS3 ELISA Kit;Human RALD ELISA Kit;Human c-Ki-ras2 ELISA Kit;Human KRAS proto-oncogene, GTPase ELISA Kit;Human GTPase KRas ELISA Kit;Human K-ras p21 protein ELISA Kit;Human Kirsten rat sarcoma viral oncogene homolog ELISA Kit;Human PR310 c-K-ras oncogene ELISA Kit;Human c-Kirsten-ras protein ELISA Kit;Human cellular c-Ki-ras2 proto-oncogene ELISA Kit;Human cellular transforming proto-oncogene ELISA Kit;Human oncogene KRAS2 ELISA Kit;Human transforming protein p21 ELISA Kit;Human v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog ELISA Kit;
Catalog
E021928
species
Human
GeneKRAS
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human KRAS. No significant cross-reactivity or interference between Human KRAS and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human KRAS and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.188ng/mL
Intended UseHuman KRAS ELISA Kit allows for the in vitro quantitative determination of KRAS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KRAS in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KRAS. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human KRAS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KRAS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human KRAS. You can calculate the concentration of Human KRAS in the samples by comparing the OD of the samples to the standard curve.
Human GTPase KRas, N-terminally processed Protein information
Uniprot ID RASK_HUMAN
Uniprot AC P01116; A8K8Z5; B0LPF9; P01118; Q96D10;
UniGene Hs.37003; Hs.505033;
GeneID 3845
KEGG hsa:3845;
Human GTPase KRas, N-terminally processed Protein SEQUENCE
SEQUENCE 189 AA; 21656 MW; 973547B2E11C2C81 CRC64;
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI
KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ
RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCIIM
UCSC uc001rgp.3; human. [P01116-1];

Category

Human KRAS ELISA Kit