Human KLF15 ELISA Kit

Alternative name
Human kidney-enriched krueppel-like factor ELISA Kit;Human KKLF ELISA Kit;Human Kruppel like factor 15 ELISA Kit;Human Krueppel-like factor 15 ELISA Kit;Human kidney-enriched Kruppel-like factor ELISA Kit;
Catalog
E021950
species
Human
GeneKLF15
SpecificityThis assay has high sensitivity and excellent specificity for detection of KLF15. No significant cross-reactivity or interference between KLF15 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human KLF15 is typically less than 0.068ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman KLF15 ELISA Kit allows for the in vitro quantitative determination of KLF15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KLF15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to KLF15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KLF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KLF15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Krueppel-like factor 15 Protein information
Uniprot ID KLF15_HUMAN
Uniprot AC Q9UIH9;
UniGene Hs.272215;
GeneID 28999
KEGG hsa:28999;
Human Krueppel-like factor 15 Protein SEQUENCE
SEQUENCE 416 AA; 43992 MW; 6335F85141BEB276 CRC64;
MVDHLLPVDE NFSSPKCPVG YLGDRLVGRR AYHMLPSPVS EDDSDASSPC
SCSSPDSQAL CSCYGGGLGT ESQDSILDFL LSQATLGSGG GSGSSIGASS
GPVAWGPWRR AAAPVKGEHF CLPEFPLGDP DDVPRPFQPT LEEIEEFLEE
NMEPGVKEVP EGNSKDLDAC SQLSAGPHKS HLHPGSSGRE RCSPPPGGAS
AGGAQGPGGG PTPDGPIPVL LQIQPVPVKQ ESGTGPASPG QAPENVKVAQ
LLVNIQGQTF ALVPQVVPSS NLNLPSKFVR IAPVPIAAKP VGSGPLGPGP
AGLLMGQKFP KNPAAELIKM HKCTFPGCSK MYTKSSHLKA HLRRHTGEKP
FACTWPGCGW RFSRSDELSR HRRSHSGVKP YQCPVCEKKF ARSDHLSKHI
KVHRFPRSSR SVRSVN
UCSC uc011bkk.2; human.;

Category

Human KLF15 ELISA Kit

Human kidney-enriched krueppel-like factor ELISA Kit;Human KKLF ELISA Kit;Human Kruppel like factor 15 ELISA Kit;Human Krueppel-like factor 15 ELISA Kit;Human kidney-enriched Kruppel-like factor ELISA Kit;