Human KLF16 ELISA Kit

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  • Alternative name

    Human Basic transcription element-binding protein 4 ELISA Kit;Human BTE-binding protein 4 ELISA Kit;Human novel Sp1-like zinc finger transcription factor 2 ELISA Kit;Human transcription factor BTEB4 ELISA Kit;Human transcription factor NSLP2 ELISA Kit;Human BTEB4 ELISA Kit;Human NSLP2 ELISA Kit;Human DRRF ELISA Kit;Human Kruppel like factor 16 ELISA Kit;Human Krueppel-like factor 16 ELISA Kit;Human basic transcription element binding protein 4 ELISA Kit;Human dopamine receptor regulating factor ELISA Kit;

  • Catalog
    E021951
  • species
    Human
  • GeneKLF16
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman KLF16 ELISA Kit allows for the in vitro quantitative determination of KLF16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the Assay: KLF16 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for KLF16. Standards or samples are then added to the microtiter plate wells and KLF16 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of KLF16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for KLF16 are added to each well to "sandwich" the KLF16 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain KLF16 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLF16 concentration in each sample is interpolated from this standard curve.
  • Human Krueppel-like factor 16 Protein information
  • Uniprot ID KLF16_HUMAN
  • Uniprot AC Q9BXK1;
  • UniGene Hs.136280;
  • GeneID 83855
  • KEGG hsa:83855;
  • Human Krueppel-like factor 16 Protein SEQUENCE
  • SEQUENCE 252 AA; 25431 MW; 9A0CB4B1A585A118 CRC64;

    MSAAVACVDY FAADVLMAIS SGAVVHRGRP GPEGAGPAAG LDVRAARREA

    ASPGTPGPPP PPPAASGPGP GAAAAPHLLA ASILADLRGG PGAAPGGASP

    ASSSSAASSP SSGRAPGAAP SAAAKSHRCP FPDCAKAYYK SSHLKSHLRT

    HTGERPFACD WQGCDKKFAR SDELARHHRT HTGEKRFSCP LCSKRFTRSD

    HLAKHARRHP GFHPDLLRRP GARSTSPSDS LPCSLAGSPA PSPAPSPAPA

    GL

  • UCSC uc002luc.4; human.;



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