Human SELL ELISA Kit

Alternative name
Human CD62 antigen-like family member L ELISA Kit;Human Leukocyte adhesion molecule 1 ELISA Kit;Human LAM-1 ELISA Kit;Human leukocyte surface antigen Leu-8 ELISA Kit;Human leukocyte-endothelial cell adhesion molecule 1 ELISA Kit;Human LECAM1 ELISA Kit;Human lymph node homing receptor ELISA Kit;Human TQ1 ELISA Kit;Human gp90-MEL ELISA Kit;Human LNHR ELISA Kit;Human LYAM1 ELISA Kit;Human CD62L ELISA Kit;Human LAM1 ELISA Kit;Human LEU8 ELISA Kit;Human LSEL ELISA Kit;Human PLNHR ELISA Kit;Human selectin L ELISA Kit;Human L-selectin ELISA Kit;Human lymphocyte adhesion molecule 1 ELISA Kit;Human pln homing receptor ELISA Kit;
Catalog
E022031
species
Human
GeneSELL
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intended Uses For detection of L-Selectin in human serum, plasma, body fluids, tissue lysat
Intended UseHuman SELL ELISA Kit allows for the in vitro quantitative determination of SELL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology; Cytokines & Cell Signalling; Research Assays; Assay Categories
Product Description
specificalPrinciple of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- L-Selectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- L-Selectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the L-Selectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of L-Selectin can be calculated. Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on lymphocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4-binding proteins. L-selectin acts as a "homing receptor" for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. It may also play a role in neutrophil adhesion to endothelium at sites of inflammation.
Human L-selectin Protein information
Uniprot ID LYAM1_HUMAN
Uniprot AC P14151; B2R6Q8; P15023; Q9UJ43;
UniGene Hs.728756;
GeneID 6402
KEGG hsa:6402;
Human L-selectin Protein SEQUENCE
SEQUENCE 372 AA; 42187 MW; 6EA9918ECA2D3643 CRC64;
MIFPWKCQST QRDLWNIFKL WGWTMLCCDF LAHHGTDCWT YHYSEKPMNW
QRARRFCRDN YTDLVAIQNK AEIEYLEKTL PFSRSYYWIG IRKIGGIWTW
VGTNKSLTEE AENWGDGEPN NKKNKEDCVE IYIKRNKDAG KWNDDACHKL
KAALCYTASC QPWSCSGHGE CVEIINNYTC NCDVGYYGPQ CQFVIQCEPL
EAPELGTMDC THPLGNFSFS SQCAFSCSEG TNLTGIEETT CGPFGNWSSP
EPTCQVIQCE PLSAPDLGIM NCSHPLASFS FTSACTFICS EGTELIGKKK
TICESSGIWS NPSPICQKLD KSFSMIKEGD YNPLFIPVAV MVTAFSGLAF
IIWLARRLKK GKKSKRSMND PY
UCSC uc001ggk.4; human. [P14151-1];

Category

Human SELL ELISA Kit