Human LMNB1 ELISA Kit

Alternative name
Human LMN2 ELISA Kit;Human LMNB ELISA Kit;Human ADLD ELISA Kit;Human LMN ELISA Kit;Human lamin B1 ELISA Kit;Human lamin-B1 ELISA Kit;
Catalog
E022135
species
Human
GeneLMNB1
SpecificityThis assay has high sensitivity for detection of LMNB1. 100% cross-reactivity of LMNB1 was observed among human, mouse and rat.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of LMNB1 is typically less than 0.055ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
Intended UseHuman LMNB1 ELISA Kit allows for the in vitro quantitative determination of LMNB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LMNB1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LMNB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LMNB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LMNB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Lamin-B1 Protein information
Uniprot ID LMNB1_HUMAN
Uniprot AC P20700; B2R6J6; Q3SYN7; Q96EI6;
UniGene Hs.89497;
GeneID 4001
KEGG hsa:4001;
Human Lamin-B1 Protein SEQUENCE
SEQUENCE 586 AA; 66408 MW; 73292877745722C4 CRC64;
MATATPVPPR MGSRAGGPTT PLSPTRLSRL QEKEELRELN DRLAVYIDKV
RSLETENSAL QLQVTEREEV RGRELTGLKA LYETELADAR RALDDTARER
AKLQIELGKC KAEHDQLLLN YAKKESDLNG AQIKLREYEA ALNSKDAALA
TALGDKKSLE GDLEDLKDQI AQLEASLAAA KKQLADETLL KVDLENRCQS
LTEDLEFRKS MYEEEINETR RKHETRLVEV DSGRQIEYEY KLAQALHEMR
EQHDAQVRLY KEELEQTYHA KLENARLSSE MNTSTVNSAR EELMESRMRI
ESLSSQLSNL QKESRACLER IQELEDLLAK EKDNSRRMLT DKEREMAEIR
DQMQQQLNDY EQLLDVKLAL DMEISAYRKL LEGEEERLKL SPSPSSRVTV
SRASSSRSVR TTRGKRKRVD VEESEASSSV SISHSASATG NVCIEEIDVD
GKFIRLKNTS EQDQPMGGWE MIRKIGDTSV SYKYTSRYVL KAGQTVTIWA
ANAGVTASPP TDLIWKNQNS WGTGEDVKVI LKNSQGEEVA QRSTVFKTTI
PEEEEEEEEA AGVVVEEELF HQQGTPRASN RSCAIM
UCSC uc003kud.3; human.;

Category

Human LMNB1 ELISA Kit

Human LMN2 ELISA Kit;Human LMNB ELISA Kit;Human ADLD ELISA Kit;Human LMN ELISA Kit;Human lamin B1 ELISA Kit;Human lamin-B1 ELISA Kit;