Human LAMB3 ELISA Kit

Alternative name
Human epiligrin subunit bata ELISA Kit;Human kalinin B1 chain ELISA Kit;Human kalinin subunit beta ELISA Kit;Human laminin B1k chain ELISA Kit;Human laminin-5 subunit beta ELISA Kit;Human nicein subunit beta ELISA Kit;Human LAMNB1 ELISA Kit;Human AI1A ELISA Kit;Human BM600-125KDA ELISA Kit;Human LAM5 ELISA Kit;Human laminin subunit beta 3 ELISA Kit;Human laminin subunit beta-3 ELISA Kit;Human kalinin-140kDa ELISA Kit;Human laminin S B3 chain ELISA Kit;Human laminin beta 3 ELISA Kit;Human laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)) ELISA Kit;Human nicein-125kDa ELISA Kit;
Catalog
E022161
species
Human
GeneLAMB3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Laminin Beta 3 (LAMb3). No significant cross-reactivity or interference between Laminin Beta 3 (LAMb3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.26ng/mL.
Intended UseHuman LAMB3 ELISA Kit allows for the in vitro quantitative determination of LAMB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Beta 3 (LAMb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Beta 3 (LAMb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Beta 3 (LAMb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Beta 3 (LAMb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Laminin subunit beta-3 Protein information
Uniprot ID LAMB3_HUMAN
Uniprot AC Q13751; D3DT88; O14947; Q14733; Q9UJK4; Q9UJL1;
UniGene Hs.497636;
GeneID 3914
KEGG hsa:3914;
Human Laminin subunit beta-3 Protein SEQUENCE
SEQUENCE 1172 AA; 129572 MW; 61BC1A60BBD4FA05 CRC64;
MRPFFLLCFA LPGLLHAQQA CSRGACYPPV GDLLVGRTRF LRASSTCGLT
KPETYCTQYG EWQMKCCKCD SRQPHNYYSH RVENVASSSG PMRWWQSQND
VNPVSLQLDL DRRFQLQEVM MEFQGPMPAG MLIERSSDFG KTWRVYQYLA
ADCTSTFPRV RQGRPQSWQD VRCQSLPQRP NARLNGGKVQ LNLMDLVSGI
PATQSQKIQE VGEITNLRVN FTRLAPVPQR GYHPPSAYYA VSQLRLQGSC
FCHGHADRCA PKPGASAGPS TAVQVHDVCV CQHNTAGPNC ERCAPFYNNR
PWRPAEGQDA HECQRCDCNG HSETCHFDPA VFAASQGAYG GVCDNCRDHT
EGKNCERCQL HYFRNRRPGA SIQETCISCE CDPDGAVPGA PCDPVTGQCV
CKEHVQGERC DLCKPGFTGL TYANPQGCHR CDCNILGSRR DMPCDEESGR
CLCLPNVVGP KCDQCAPYHW KLASGQGCEP CACDPHNSLS PQCNQFTGQC
PCREGFGGLM CSAAAIRQCP DRTYGDVATG CRACDCDFRG TEGPGCDKAS
GRCLCRPGLT GPRCDQCQRG YCNRYPVCVA CHPCFQTYDA DLREQALRFG
RLRNATASLW SGPGLEDRGL ASRILDAKSK IEQIRAVLSS PAVTEQEVAQ
VASAILSLRR TLQGLQLDLP LEEETLSLPR DLESLDRSFN GLLTMYQRKR
EQFEKISSAD PSGAFRMLST AYEQSAQAAQ QVSDSSRLLD QLRDSRREAE
RLVRQAGGGG GTGSPKLVAL RLEMSSLPDL TPTFNKLCGN SRQMACTPIS
CPGELCPQDN GTACGSRCRG VLPRAGGAFL MAGQVAEQLR GFNAQLQRTR
QMIRAAEESA SQIQSSAQRL ETQVSASRSQ MEEDVRRTRL LIQQVRDFLT
DPDTDAATIQ EVSEAVLALW LPTDSATVLQ KMNEIQAIAA RLPNVDLVLS
QTKQDIARAR RLQAEAEEAR SRAHAVEGQV EDVVGNLRQG TVALQEAQDT
MQGTSRSLRL IQDRVAEVQQ VLRPAEKLVT SMTKQLGDFW TRMEELRHQA
RQQGAEAVQA QQLAEGASEQ ALSAQEGFER IKQKYAELKD RLGQSSMLGE
QGARIQSVKT EAEELFGETM EMMDRMKDME LELLRGSQAI MLRSADLTGL
EKRVEQIRDH INGRVLYYAT CK
UCSC uc001hhg.4; human.;

Category

Human LAMB3 ELISA Kit