Human LRG1 ELISA Kit

Alternative name
Human LRG ELISA Kit;Human HMFT1766 ELISA Kit;Human leucine rich alpha-2-glycoprotein 1 ELISA Kit;Human leucine-rich alpha-2-glycoprotein ELISA Kit;Human 1300008B03Rik ELISA Kit;Human 2310031E04Rik ELISA Kit;Human leucine rich alpha 2 glycoprotein ELISA Kit;
Catalog
E022398
species
Human
GeneLRG1
SpecificityThis assay has high sensitivity and excellent specificity for detection of LRG1. No significant cross-reactivity or interference between LRG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LRG1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman LRG1 ELISA Kit allows for the in vitro quantitative determination of LRG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This LRG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LRG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||LRG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LRG1 antibody and an LRG1-HRP conjugate. The assay sample and buffer are incubated together with LRG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LRG1 concentration since LRG1 from samples and LRG1-HRP conjugate compete for the anti-LRG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LRG1 from the sample, fewer sites are left to bind LRG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRG1 concentration in each sample is interpolated from this standard curve.
Human Leucine-rich alpha-2-glycoprotein Protein information
Uniprot ID A2GL_HUMAN
Uniprot AC P02750; Q8N4F5; Q96QZ4;
UniGene Hs.655559;
GeneID 116844
KEGG hsa:116844;
Human Leucine-rich alpha-2-glycoprotein Protein SEQUENCE
SEQUENCE 347 AA; 38178 MW; 20C99ED50152FA9C CRC64;
MSSWSRQRPK SPGGIQPHVS RTLFLLLLLA ASAWGVTLSP KDCQVFRSDH
GSSISCQPPA EIPGYLPADT VHLAVEFFNL THLPANLLQG ASKLQELHLS
SNGLESLSPE FLRPVPQLRV LDLTRNALTG LPPGLFQASA TLDTLVLKEN
QLEVLEVSWL HGLKALGHLD LSGNRLRKLP PGLLANFTLL RTLDLGENQL
ETLPPDLLRG PLQLERLHLE GNKLQVLGKD LLLPQPDLRY LFLNGNKLAR
VAAGAFQGLR QLDMLDLSNN SLASVPEGLW ASLGQPNWDM RDGFDISGNP
WICDQNLSDL YRWLQAQKDK MFSQNDTRCA GPEAVKGQTL LAVAKSQ
UCSC uc002mau.4; human.;

Category

Human LRG1 ELISA Kit

Human LRG ELISA Kit;Human HMFT1766 ELISA Kit;Human leucine rich alpha-2-glycoprotein 1 ELISA Kit;Human leucine-rich alpha-2-glycoprotein ELISA Kit;Human 1300008B03Rik ELISA Kit;Human 2310031E04Rik ELISA Kit;Human leucine rich alpha 2 glycoprotein ELISA Kit;