Human SERPINB1 ELISA Kit

Alternative name
Human LEI ELISA Kit;Human Monocyte/neutrophil elastase inhibitor ELISA Kit;Human EI ELISA Kit;Human M/NEI ELISA Kit;Human peptidase inhibitor 2 ELISA Kit;Human PI-2 ELISA Kit;Human Serpin B1 ELISA Kit;Human ELANH2 ELISA Kit;Human MNEI ELISA Kit;Human PI2 ELISA Kit;Human HEL-S-27 ELISA Kit;Human HEL57 ELISA Kit;Human serpin family B member 1 ELISA Kit;Human leukocyte elastase inhibitor ELISA Kit;Human epididymis luminal protein 57 ELISA Kit;Human epididymis secretory protein Li 27 ELISA Kit;Human protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived ELISA Kit;Human serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1 ELISA Kit;Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 ELISA Kit;
Catalog
E022607
species
Human
GeneSERPINB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of SERPINB1. No significant cross-reactivity or interference between SERPINB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SERPINB1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman SERPINB1 ELISA Kit allows for the in vitro quantitative determination of SERPINB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalIntended Uses: This SERPINB1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SERPINB1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||SERPINB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SERPINB1 antibody and an SERPINB1-HRP conjugate. The assay sample and buffer are incubated together with SERPINB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SERPINB1 concentration since SERPINB1 from samples and SERPINB1-HRP conjugate compete for the anti-SERPINB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SERPINB1 from the sample, fewer sites are left to bind SERPINB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SERPINB1 concentration in each sample is interpolated from this standard curve.
Human Leukocyte elastase inhibitor Protein information
Uniprot ID ILEU_HUMAN
Uniprot AC P30740; A8K5L2; B4DNT0; Q53FB9; Q5W0E1; Q9UDF8;
UniGene Hs.381167;
GeneID 1992
KEGG hsa:1992;
Human Leukocyte elastase inhibitor Protein SEQUENCE
SEQUENCE 379 AA; 42742 MW; BAAE08DFCBCD8CD3 CRC64;
MEQLSSANTR FALDLFLALS ENNPAGNIFI SPFSISSAMA MVFLGTRGNT
AAQLSKTFHF NTVEEVHSRF QSLNADINKR GASYILKLAN RLYGEKTYNF
LPEFLVSTQK TYGADLASVD FQHASEDARK TINQWVKGQT EGKIPELLAS
GMVDNMTKLV LVNAIYFKGN WKDKFMKEAT TNAPFRLNKK DRKTVKMMYQ
KKKFAYGYIE DLKCRVLELP YQGEELSMVI LLPDDIEDES TGLKKIEEQL
TLEKLHEWTK PENLDFIEVN VSLPRFKLEE SYTLNSDLAR LGVQDLFNSS
KADLSGMSGA RDIFISKIVH KSFVEVNEEG TEAAAATAGI ATFCMLMPEE
NFTADHPFLF FIRHNSSGSI LFLGRFSSP

Category

Human SERPINB1 ELISA Kit