Human ACAT2 ELISA Kit

Alternative name
Human acetyl-CoA transferase-like protein ELISA Kit;Human cytosolic acetoacetyl-CoA thiolase ELISA Kit;Human ACTL ELISA Kit;Human acetyl-CoA acetyltransferase 2 ELISA Kit;Human acetyl-CoA acetyltransferase, cytosolic ELISA Kit;Human acetoacetyl Coenzyme A thiolase ELISA Kit;
Catalog
E000227
species
Human
GeneACAT2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). No significant cross-reactivity or interference between Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 31.5pg/mL.
Intended UseHuman ACAT2 ELISA Kit allows for the in vitro quantitative determination of ACAT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 2 (ACAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Acetyl-CoA acetyltransferase, cytosolic Protein information
Uniprot ID THIC_HUMAN
Uniprot AC Q9BWD1; B7Z233; E1P5B1; Q16146; Q5TCL7; Q8TDM4;
UniGene Hs.571037;
GeneID 39
KEGG hsa:39;
Human Acetyl-CoA acetyltransferase, cytosolic Protein SEQUENCE
SEQUENCE 397 AA; 41351 MW; E3A8DAFB6F341B18 CRC64;
MNAGSDPVVI VSAARTIIGS FNGALAAVPV QDLGSTVIKE VLKRATVAPE
DVSEVIFGHV LAAGCGQNPV RQASVGAGIP YSVPAWSCQM ICGSGLKAVC
LAVQSIGIGD SSIVVAGGME NMSKAPHLAY LRTGVKIGEM PLTDSILCDG
LTDAFHNCHM GITAENVAKK WQVSREDQDK VAVLSQNRTE NAQKAGHFDK
EIVPVLVSTR KGLIEVKTDE FPRHGSNIEA MSKLKPYFLT DGTGTVTPAN
ASGINDGAAA VVLMKKSEAD KRGLTPLARI VSWSQVGVEP SIMGIGPIPA
IKQAVTKAGW SLEDVDIFEI NEAFAAVSAA IVKELGLNPE KVNIEGGAIA
LGHPLGASGC RILVTLLHTL ERMGRSRGVA ALCIGGGMGI AMCVQRE
UCSC uc010kjy.4; human. [Q9BWD1-1];

Category

Human ACAT2 ELISA Kit

Human acetyl-CoA transferase-like protein ELISA Kit;Human cytosolic acetoacetyl-CoA thiolase ELISA Kit;Human ACTL ELISA Kit;Human acetyl-CoA acetyltransferase 2 ELISA Kit;Human acetyl-CoA acetyltransferase, cytosolic ELISA Kit;Human acetoacetyl Coenzyme A thiolase ELISA Kit;