Human LIPH ELISA Kit

Alternative name
Human LIPH ELISA Kit;Human LPD lipase-related protein ELISA Kit;Human membrane-associated phosphatidic acid-selective phospholipase A1-alpha ELISA Kit;Human mPA-PLA1 alpha ELISA Kit;Human phospholipase A1 member B ELISA Kit;Human LPDLR ELISA Kit;Human MPAPLA1 ELISA Kit;Human PLA1B ELISA Kit;Human AH ELISA Kit;Human ARWH2 ELISA Kit;Human HYPT7 ELISA Kit;Human LAH2 ELISA Kit;Human mPA-PLA1 ELISA Kit;Human lipase H ELISA Kit;Human lipase member H ELISA Kit;Human lipase, member H ELISA Kit;Human membrane-bound phosphatidic acid-selective phospholipase A1 ELISA Kit;Human phospholipase A(1) ELISA Kit;
Catalog
E022769
species
Human
GeneLIPH
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase H (LIPH). No significant cross-reactivity or interference between Lipase H (LIPH) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.68ng/mL.
Intended UseHuman LIPH ELISA Kit allows for the in vitro quantitative determination of LIPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase H (LIPH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase H (LIPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase H (LIPH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase H (LIPH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Lipase member H Protein information
Uniprot ID LIPH_HUMAN
Uniprot AC Q8WWY8; A2IBA7; Q8TEC7;
UniGene Hs.68864;
GeneID 200879
KEGG hsa:200879;
Human Lipase member H Protein SEQUENCE
SEQUENCE 451 AA; 50859 MW; 949CE32B0C15868B CRC64;
MLRFYLFISL LCLSRSDAEE TCPSFTRLSF HSAVVGTGLN VRLMLYTRKN
LTCAQTINSS AFGNLNVTKK TTFIVHGFRP TGSPPVWMDD LVKGLLSVED
MNVVVVDWNR GATTLIYTHA SSKTRKVAMV LKEFIDQMLA EGASLDDIYM
IGVSLGAHIS GFVGEMYDGW LGRITGLDPA GPLFNGKPHQ DRLDPSDAQF
VDVIHSDTDA LGYKEPLGNI DFYPNGGLDQ PGCPKTILGG FQYFKCDHQR
SVYLYLSSLR ESCTITAYPC DSYQDYRNGK CVSCGTSQKE SCPLLGYYAD
NWKDHLRGKD PPMTKAFFDT AEESPFCMYH YFVDIITWNK NVRRGDITIK
LRDKAGNTTE SKINHEPTTF QKYHQVSLLA RFNQDLDKVA AISLMFSTGS
LIGPRYKLRI LRMKLRSLAH PERPQLCRYD LVLMENVETV FQPILCPELQ
L
UCSC uc003fpm.4; human.;

Category

Human LIPH ELISA Kit