Human LIPC ELISA Kit

Alternative name
Human HL ELISA Kit;Human hepatic lipase ELISA Kit;Human lipase member C ELISA Kit;Human HTGL ELISA Kit;Human HDLCQ12 ELISA Kit;Human LIPH ELISA Kit;Human lipase C, hepatic type ELISA Kit;Human hepatic triacylglycerol lipase ELISA Kit;Human Triacylglycerol lipase ELISA Kit;Human lipase, hepatic ELISA Kit;
Catalog
E022785
species
Human
GeneLIPC
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase, Hepatic (LIPC). No significant cross-reactivity or interference between Lipase, Hepatic (LIPC) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 33pg/mL.
Intended UseHuman LIPC ELISA Kit allows for the in vitro quantitative determination of LIPC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hepatic (LIPC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hepatic (LIPC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hepatic (LIPC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Hepatic (LIPC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Hepatic triacylglycerol lipase Protein information
Uniprot ID LIPC_HUMAN
Uniprot AC P11150; A2RUB4; A8K9B6; O43571; P78529; Q99465;
UniGene Hs.654472;
GeneID 3990
KEGG hsa:3990;
Human Hepatic triacylglycerol lipase Protein SEQUENCE
SEQUENCE 499 AA; 55914 MW; 1FC7567CAE3E514B CRC64;
MDTSPLCFSI LLVLCIFIQS SALGQSLKPE PFGRRAQAVE TNKTLHEMKT
RFLLFGETNQ GCQIRINHPD TLQECGFNSS LPLVMIIHGW SVDGVLENWI
WQMVAALKSQ PAQPVNVGLV DWITLAHDHY TIAVRNTRLV GKEVAALLRW
LEESVQLSRS HVHLIGYSLG AHVSGFAGSS IGGTHKIGRI TGLDAAGPLF
EGSAPSNRLS PDDANFVDAI HTFTREHMGL SVGIKQPIGH YDFYPNGGSF
QPGCHFLELY RHIAQHGFNA ITQTIKCSHE RSVHLFIDSL LHAGTQSMAY
PCGDMNSFSQ GLCLSCKKGR CNTLGYHVRQ EPRSKSKRLF LVTRAQSPFK
VYHYQFKIQF INQTETPIQT TFTMSLLGTK EKMQKIPITL GKGIASNKTY
SFLITLDVDI GELIMIKFKW ENSAVWANVW DTVQTIIPWS TGPRHSGLVL
KTIRVKAGET QQRMTFCSEN TDDLLLRPTQ EKIFVKCEIK SKTSKRKIR
UCSC uc002afa.3; human.;

Category

Human LIPC ELISA Kit