Human LIPE ELISA Kit

Alternative name
Human HSL ELISA Kit;Human AOMS4 ELISA Kit;Human FPLD6 ELISA Kit;Human LHS ELISA Kit;Human lipase E, hormone sensitive type ELISA Kit;Human hormone-sensitive lipase ELISA Kit;Human hormone-sensitive lipase testicular isoform ELISA Kit;Human lipase, hormone-sensitive ELISA Kit;
Catalog
E022786
species
Human
GeneLIPE
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase, Hormone Sensitive (LIPE). No significant cross-reactivity or interference between Lipase, Hormone Sensitive (LIPE) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.065ng/mL.
Intended UseHuman LIPE ELISA Kit allows for the in vitro quantitative determination of LIPE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hormone Sensitive (LIPE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hormone Sensitive (LIPE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hormone Sensitive (LIPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Hormone Sensitive (LIPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Hormone-sensitive lipase Protein information
Uniprot ID LIPS_HUMAN
Uniprot AC Q05469; Q3LRT2; Q6NSL7;
UniGene Hs.656980;
GeneID 3991
KEGG hsa:3991;
Human Hormone-sensitive lipase Protein SEQUENCE
SEQUENCE 1076 AA; 116598 MW; 1CC0E3880FF64D74 CRC64;
MEPGSKSVSR SDWQPEPHQR PITPLEPGPE KTPIAQPESK TLQGSNTQQK
PASNQRPLTQ QETPAQHDAE SQKEPRAQQK SASQEEFLAP QKPAPQQSPY
IQRVLLTQQE AASQQGPGLG KESITQQEPA LRQRHVAQPG PGPGEPPPAQ
QEAESTPAAQ AKPGAKREPS APTESTSQET PEQSDKQTTP VQGAKSKQGS
LTELGFLTKL QELSIQRSAL EWKALSEWVT DSESESDVGS SSDTDSPATM
GGMVAQGVKL GFKGKSGYKV MSGYSGTSPH EKTSARNHRH YQDTASRLIH
NMDLRTMTQS LVTLAEDNIA FFSSQGPGET AQRLSGVFAG VREQALGLEP
ALGRLLGVAH LFDLDPETPA NGYRSLVHTA RCCLAHLLHK SRYVASNRRS
IFFRTSHNLA ELEAYLAALT QLRALVYYAQ RLLVTNRPGV LFFEGDEGLT
ADFLREYVTL HKGCFYGRCL GFQFTPAIRP FLQTISIGLV SFGEHYKRNE
TGLSVAASSL FTSGRFAIDP ELRGAEFERI TQNLDVHFWK AFWNITEMEV
LSSLANMASA TVRVSRLLSL PPEAFEMPLT ADPTLTVTIS PPLAHTGPGP
VLVRLISYDL REGQDSEELS SLIKSNGQRS LELWPRPQQA PRSRSLIVHF
HGGGFVAQTS RSHEPYLKSW AQELGAPIIS IDYSLAPEAP FPRALEECFF
AYCWAIKHCA LLGSTGERIC LAGDSAGGNL CFTVALRAAA YGVRVPDGIM
AAYPATMLQP AASPSRLLSL MDPLLPLSVL SKCVSAYAGA KTEDHSNSDQ
KALGMMGLVR RDTALLLRDF RLGASSWLNS FLELSGRKSQ KMSEPIAEPM
RRSVSEAALA QPQGPLGTDS LKNLTLRDLS LRGNSETSSD TPEMSLSAET
LSPSTPSDVN FLLPPEDAGE EAEAKNELSP MDRGLGVRAA FPEGFHPRRS
SQGATQMPLY SSPIVKNPFM SPLLAPDSML KSLPPVHIVA CALDPMLDDS
VMLARRLRNL GQPVTLRVVE DLPHGFLTLA ALCRETRQAA ELCVERIRLV
LTPPAGAGPS GETGAAGVDG GCGGRH
UCSC uc002otr.4; human. [Q05469-1];

Category

Human LIPE ELISA Kit

Human HSL ELISA Kit;Human AOMS4 ELISA Kit;Human FPLD6 ELISA Kit;Human LHS ELISA Kit;Human lipase E, hormone sensitive type ELISA Kit;Human hormone-sensitive lipase ELISA Kit;Human hormone-sensitive lipase testicular isoform ELISA Kit;Human lipase, hormone-sensitive ELISA Kit;