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Human LCN2 ELISA Kit

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  • Alternative name

    Human NGAL ELISA Kit;Human 25 kDa alpha-2-microglobulin-related subunit of MMP-9 ELISA Kit;Human Lipocalin-2 ELISA Kit;Human oncogene 24p3 ELISA Kit;Human siderocalin LCN2 ELISA Kit;Human p25 ELISA Kit;Human HNL ELISA Kit;Human 24p3 ELISA Kit;Human MSFI ELISA Kit;Human lipocalin 2 ELISA Kit;Human neutrophil gelatinase-associated lipocalin ELISA Kit;Human migration-stimulating factor inhibitor ELISA Kit;
  • Catalog
    E022833
  • species
    Human
  • GeneLCN2
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Intended Uses For detection of Lipocalin-2 in human serum, plasma, urine, cell culture supe
  • Intended UseHuman LCN2 ELISA Kit allows for the in vitro quantitative determination of LCN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology; Cytokines & Cell Signalling; Research Assays; Assay Categories
  • Product Description
    specifical    Principle of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-NGAL antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-NGAL antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NGAL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NGAL can be calculated. Background: Lipocalin-2 (LCN2), also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), is a protein associated with neutrophil gelatinase. The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.
  • Human Neutrophil gelatinase-associated lipocalin Protein information
  • Uniprot ID NGAL_HUMAN
  • Uniprot AC Q92683;
  • UniGene Hs.204238;
  • GeneID 3934
  • KEGG hsa:3934;
  • Human Neutrophil gelatinase-associated lipocalin Protein SEQUENCE

  • SEQUENCE 198 AA; 22588 MW; CD761805723FEF1E CRC64;

    MPLGLLWLGL ALLGALHAQA QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK

    WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI

    RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR

    EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG

  • UCSC uc004bto.1; human. [P80188-1];



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