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Human LIAS ELISA Kit

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  • Alternative name

    Human lipoate synthase ELISA Kit;Human LS ELISA Kit;Human lip-syn ELISA Kit;Human Lipoic acid synthase ELISA Kit;Human LAS ELISA Kit;Human HGCLAS ELISA Kit;Human HUSSY-01 ELISA Kit;Human LIP1 ELISA Kit;Human PDHLD ELISA Kit;Human lipoic acid synthetase ELISA Kit;Human lipoyl synthase, mitochondrial ELISA Kit;
  • Catalog
    E022837
  • species
    Human
  • GeneLIAS
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human LIAS. No significant cross-reactivity or interference between human LIAS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human LIAS is typically less than 8.59 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman LIAS ELISA Kit allows for the in vitro quantitative determination of LIAS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LIAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIAS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIAS bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Lipoyl synthase, mitochondrial Protein information
  • Uniprot ID LIAS_HUMAN
  • Uniprot AC O43766; A8K873; C9JCF6; Q8IV62;
  • UniGene Hs.550502;
  • GeneID 11019
  • KEGG hsa:11019;
  • Human Lipoyl synthase, mitochondrial Protein SEQUENCE

  • SEQUENCE 372 AA; 41911 MW; A5BEACA1F36CEB74 CRC64;

    MSLRCGDAAR TLGPRVFGRY FCSPVRPLSS LPDKKKELLQ NGPDLQDFVS

    GDLADRSTWD EYKGNLKRQK GERLRLPPWL KTEIPMGKNY NKLKNTLRNL

    NLHTVCEEAR CPNIGECWGG GEYATATATI MLMGDTCTRG CRFCSVKTAR

    NPPPLDASEP YNTAKAIAEW GLDYVVLTSV DRDDMPDGGA EHIAKTVSYL

    KERNPKILVE CLTPDFRGDL KAIEKVALSG LDVYAHNVET VPELQSKVRD

    PRANFDQSLR VLKHAKKVQP DVISKTSIML GLGENDEQVY ATMKALREAD

    VDCLTLGQYM QPTRRHLKVE EYITPEKFKY WEKVGNELGF HYTASGPLVR

    SSYKAGEFFL KNLVAKRKTK DL

  • UCSC uc003guf.5; human. [O43766-1];



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