Human OLR1 ELISA Kit

Alternative name
Human Ox-LDL receptor 1 ELISA Kit;Human C-type lectin domain family 8 member A ELISA Kit;Human Lectin-like oxidized LDL receptor 1 ELISA Kit;Human LOX-1 ELISA Kit;Human Lectin-like oxLDL receptor 1 ELISA Kit;Human hLOX-1 ELISA Kit;Human lectin-type oxidized LDL receptor 1 ELISA Kit;Human CLEC8A ELISA Kit;Human LOX1 ELISA Kit;Human LOXIN ELISA Kit;Human SCARE1 ELISA Kit;Human SLOX1 ELISA Kit;Human oxidized low density lipoprotein receptor 1 ELISA Kit;Human oxidized low-density lipoprotein receptor 1 ELISA Kit;Human ox LDL receptor 1 ELISA Kit;Human oxidized low density lipoprotein (lectin-like) receptor 1 ELISA Kit;Human oxidized low-density lipoprotein receptor 1, soluble form ELISA Kit;Human scavenger receptor class E, member 1 ELISA Kit;
Catalog
E023020
species
Human
GeneOLR1
SpecificityNatural and recombinant human LOX-1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman OLR1 ELISA Kit allows for the in vitro quantitative determination of OLR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The human LOX-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LOX-1 has been precoated onto 96-well plates. Standards(NSO, S61-Q273) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOX-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LOX-1 amount of sample captured in plate. Background/Introduction: LOX-1 (lectin-type oxidized LDL receptor 1) also known as OLR1, is a protein that in humans is encoded by the OLR1 gene. LOX-1 is a receptor protein which belongs to the C-type lectin superfamily. The LOX1 gene is mapped to 12p13-p12 by fluorescence in situ hybridization. LOX1 is expressed on the plasma membrane of differentiated macrophages, but not on monocytes. The LOX1 protein acts as a macrophage scavenger receptor. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD.
Human Oxidized low-density lipoprotein receptor 1, soluble form Protein information
Uniprot ID OLR1_HUMAN
Uniprot AC P78380; A8K7V9; B4DI48; G3V1I4; Q2PP00; Q7Z484;
UniGene Hs.412484;
GeneID 4973
KEGG hsa:4973;
Human Oxidized low-density lipoprotein receptor 1, soluble form Protein SEQUENCE
SEQUENCE 273 AA; 30959 MW; 852DE6595DC3D361 CRC64;
MTFDDLKIQT VKDQPDEKSN GKKAKGLQFL YSPWWCLAAA TLGVLCLGLV
VTIMVLGMQL SQVSDLLTQE QANLTHQKKK LEGQISARQQ AEEASQESEN
ELKEMIETLA RKLNEKSKEQ MELHHQNLNL QETLKRVANC SAPCPQDWIW
HGENCYLFSS GSFNWEKSQE KCLSLDAKLL KINSTADLDF IQQAISYSSF
PFWMGLSRRN PSYPWLWEDG SPLMPHLFRV RGAVSQTYPS GTCAYIQRGA
VYAENCILAA FSICQKKANL RAQ
UCSC uc001qxo.2; human. [P78380-1];

Category

Human OLR1 ELISA Kit