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Human LYPD3 ELISA Kit

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  • Alternative name

    Human GPI-anchored metastasis-associated protein C4.4A homolog ELISA Kit;Human matrigel-induced gene C4 protein ELISA Kit;Human MIG-C4 ELISA Kit;Human C4.4A ELISA Kit;Human LY6/PLAUR domain containing 3 ELISA Kit;Human ly6/PLAUR domain-containing protein 3 ELISA Kit;Human 2310061G07Rik ELISA Kit;Human GPI-anchored metastasis-associated protein homolog ELISA Kit;
  • Catalog
    E023103
  • species
    Human
  • GeneLYPD3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LYPD3. No significant cross-reactivity or interference between LYPD3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LYPD3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman LYPD3 ELISA Kit allows for the in vitro quantitative determination of LYPD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the assay: LYPD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LYPD3 antibody and an LYPD3-HRP conjugate. The assay sample and buffer are incubated together with LYPD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LYPD3 concentration since LYPD3 from samples and LYPD3-HRP conjugate compete for the anti-LYPD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by LYPD3 from the sample, fewer sites are left to bind LYPD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LYPD3 concentration in each sample is interpolated from this standard curve.
  • Human Ly6/PLAUR domain-containing protein 3 Protein information
  • Uniprot ID LYPD3_HUMAN
  • Uniprot AC O95274; Q9UJ74;
  • UniGene Hs.631594;
  • GeneID 27076
  • KEGG hsa:27076;
  • Human Ly6/PLAUR domain-containing protein 3 Protein SEQUENCE

  • SEQUENCE 346 AA; 35971 MW; 97FF9B4A554934FF CRC64;

    MDPARKAGAQ AMIWTAGWLL LLLLRGGAQA LECYSCVQKA DDGCSPNKMK

    TVKCAPGVDV CTEAVGAVET IHGQFSLAVR GCGSGLPGKN DRGLDLHGLL

    AFIQLQQCAQ DRCNAKLNLT SRALDPAGNE SAYPPNGVEC YSCVGLSREA

    CQGTSPPVVS CYNASDHVYK GCFDGNVTLT AANVTVSLPV RGCVQDEFCT

    RDGVTGPGFT LSGSCCQGSR CNSDLRNKTY FSPRIPPLVR LPPPEPTTVA

    STTSVTTSTS APVRPTSTTK PMPAPTSQTP RQGVEHEASR DEEPRLTGGA

    AGHQDRSNSG QYPAKGGPQQ PHNKGCVAPT AGLAALLLAV AAGVLL

  • UCSC uc002owl.2; human.;



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