Human LY75 ELISA Kit

Alternative name
Human LY-75 ELISA Kit;Human C-type lectin domain family 13 member B ELISA Kit;Human DEC-205 ELISA Kit;Human GP200-MR6 ELISA Kit;Human CD205 ELISA Kit;Human CLEC13B ELISA Kit;Human lymphocyte antigen 75 ELISA Kit;
Catalog
E023111
species
Human
GeneLY75
SpecificityThis assay has high sensitivity and excellent specificity for detection of LY75 . No significant cross-reactivity or interference between LY75 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseHuman LY75 ELISA Kit allows for the in vitro quantitative determination of LY75 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LY75antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LY75 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LY75 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LY75 can be calculated.
Human Lymphocyte antigen 75 Protein information
Uniprot ID LY75_HUMAN
Uniprot AC DTKLEWVC QIPKGRTPKT PDWYNPDRAG IHGPPLIIEG SEYWFVADLH LNYEEAVLYC
UniGene Hs.153563;
GeneID 4065
KEGG hsa:4065;
Human Lymphocyte antigen 75 Protein SEQUENCE
SEQUENCE 1722 AA; 198311 MW; 4BC17EC646BF016F CRC64;
MRTGWATPRR PAGLLMLLFW FFDLAEPSGR AANDPFTIVH GNTGKCIKPV
YGWIVADDCD ETEDKLWKWV SQHRLFHLHS QKCLGLDITK SVNELRMFSC
DSSAMLWWKC EHHSLYGAAR YRLALKDGHG TAISNASDVW KKGGSEESLC
DQPYHEIYTR DGNSYGRPCE FPFLIDGTWH HDCILDEDHS GPWCATTLNY
EYDRKWGICL KPENGCEDNW EKNEQFGSCY QFNTQTALSW KEAYVSCQNQ
GADLLSINSA AELTYLKEKE GIAKIFWIGL NQLYSARGWE WSDHKPLNFL
NWDPDRPSAP TIGGSSCARM DAESGLWQSF SCEAQLPYVC RKPLNNTVEL
TDVWTYSDTR CDAGWLPNNG FCYLLVNESN SWDKAHAKCK AFSSDLISIH
SLADVEVVVT KLHNEDIKEE VWIGLKNINI PTLFQWSDGT EVTLTYWDEN
EPNVPYNKTP NCVSYLGELG QWKVQSCEEK LKYVCKRKGE KLNDASSDKM
CPPDEGWKRH GETCYKIYED EVPFGTNCNL TITSRFEQEY LNDLMKKYDK
SLRKYFWTGL RDVDSCGEYN WATVGGRRRA VTFSNWNFLE PASPGGCVAM
STGKSVGKWE VKDCRSFKAL SICKKMSGPL GPEEASPKPD DPCPEGWQSF
PASLSCYKVF HAERIVRKRN WEEAERFCQA LGAHLSSFSH VDEIKEFLHF
LTDQFSGQHW LWIGLNKRSP DLQGSWQWSD RTPVSTIIMP NEFQQDYDIR
DCAAVKVFHR PWRRGWHFYD DREFIYLRPF ACDTKLEWVC QIPKGRTPKT
PDWYNPDRAG IHGPPLIIEG SEYWFVADLH LNYEEAVLYC ASNHSFLATI
TSFVGLKAIK NKIANISGDG QKWWIRISEW PIDDHFTYSR YPWHRFPVTF
GEECLYMSAK TWLIDLGKPT DCSTKLPFIC EKYNVSSLEK YSPDSAAKVQ
CSEQWIPFQN KCFLKIKPVS LTFSQASDTC HSYGGTLPSV LSQIEQDFIT
SLLPDMEATL WIGLRWTAYE KINKWTDNRE LTYSNFHPLL VSGRLRIPEN
FFEEESRYHC ALILNLQKSP FTGTWNFTSC SERHFVSLCQ KYSEVKSRQT
LQNASETVKY LNNLYKIIPK TLTWHSAKRE CLKSNMQLVS ITDPYQQAFL
SVQALLHNSS LWIGLFSQDD ELNFGWSDGK RLHFSRWAET NGQLEDCVVL
DTDGFWKTVD CNDNQPGAIC YYSGNETEKE VKPVDSVKCP SPVLNTPWIP
FQNCCYNFII TKNRHMATTQ DEVHTKCQKL NPKSHILSIR DEKENNFVLE
QLLYFNYMAS WVMLGITYRN KSLMWFDKTP LSYTHWRAGR PTIKNEKFLA
GLSTDGFWDI QTFKVIEEAV YFHQHSILAC KIEMVDYKEE YNTTLPQFMP
YEDGIYSVIQ KKVTWYEALN MCSQSGGHLA SVHNQNGQLF LEDIVKRDGF
PLWVGLSSHD GSESSFEWSD GSTFDYIPWK GQTSPGNCVL LDPKGTWKHE
KCNSVKDGAI CYKPTKSKKL SRLTYSSRCP AAKENGSRWI QYKGHCYKSD
QALHSFSEAK KLCSKHDHSA TIVSIKDEDE NKFVSRLMRE NNNITMRVWL
GLSQHSVDQS WSWLDGSEVT FVKWENKSKS GVGRCSMLIA SNETWKKVEC
EHGFGRVVCK VPLGPDYTAI AIIVATLSIL VLMGGLIWFL FQRHRLHLAG
FSSVRYAQGV NEDEIMLPSF HD
UCSC uc002ubc.6; human. [O60449-1];

Category

Human LY75 ELISA Kit

Human LY-75 ELISA Kit;Human C-type lectin domain family 13 member B ELISA Kit;Human DEC-205 ELISA Kit;Human GP200-MR6 ELISA Kit;Human CD205 ELISA Kit;Human CLEC13B ELISA Kit;Human lymphocyte antigen 75 ELISA Kit;