Human LAG3 ELISA Kit

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  • Alternative name

    Human LAG-3 ELISA Kit;Human Protein FDC ELISA Kit;Human FDC ELISA Kit;Human CD223 ELISA Kit;Human lymphocyte activating 3 ELISA Kit;Human lymphocyte activation gene 3 protein ELISA Kit;Human lymphocyte-activation gene 3 ELISA Kit;

  • Catalog
    E023117
  • species
    Human
  • GeneLAG3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Lymphocyte Activation Gene 3 (LAG3). No significant cross-reactivity or interference between Lymphocyte Activation Gene 3 (LAG3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.056ng/mL.
  • Intended UseHuman LAG3 ELISA Kit allows for the in vitro quantitative determination of LAG3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Activation Gene 3 (LAG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Activation Gene 3 (LAG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Activation Gene 3 (LAG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Activation Gene 3 (LAG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Lymphocyte activation gene 3 protein Protein information
  • Uniprot ID LAG3_HUMAN
  • Uniprot AC P18627; A8K7T9; Q7Z643;
  • UniGene Hs.409523;
  • GeneID 3902
  • KEGG hsa:3902;
  • Human Lymphocyte activation gene 3 protein Protein SEQUENCE
  • SEQUENCE 525 AA; 57449 MW; 5E0BDEF20E9E36D2 CRC64;

    MWEAQFLGLL FLQPLWVAPV KPLQPGAEVP VVWAQEGAPA QLPCSPTIPL

    QDLSLLRRAG VTWQHQPDSG PPAAAPGHPL APGPHPAAPS SWGPRPRRYT

    VLSVGPGGLR SGRLPLQPRV QLDERGRQRG DFSLWLRPAR RADAGEYRAA

    VHLRDRALSC RLRLRLGQAS MTASPPGSLR ASDWVILNCS FSRPDRPASV

    HWFRNRGQGR VPVRESPHHH LAESFLFLPQ VSPMDSGPWG CILTYRDGFN

    VSIMYNLTVL GLEPPTPLTV YAGAGSRVGL PCRLPAGVGT RSFLTAKWTP

    PGGGPDLLVT GDNGDFTLRL EDVSQAQAGT YTCHIHLQEQ QLNATVTLAI

    ITVTPKSFGS PGSLGKLLCE VTPVSGQERF VWSSLDTPSQ RSFSGPWLEA

    QEAQLLSQPW QCQLYQGERL LGAAVYFTEL SSPGAQRSGR APGALPAGHL

    LLFLILGVLS LLLLVTGAFG FHLWRRQWRP RRFSALEQGI HPPQAQSKIE

    ELEQEPEPEP EPEPEPEPEP EPEQL

  • UCSC uc001qqs.4; human. [P18627-1];



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