Human KDM4A ELISA Kit

Alternative name
Human jmjC domain-containing histone demethylation protein 3A ELISA Kit;Human jumonji domain-containing protein 2A ELISA Kit;Human JHDM3A ELISA Kit;Human JMJD2 ELISA Kit;Human JMJD2A ELISA Kit;Human KIAA0677 ELISA Kit;Human TDRD14A ELISA Kit;Human lysine demethylase 4A ELISA Kit;Human lysine-specific demethylase 4A ELISA Kit;Human jumonji C domain-containing histone demethylase 3A ELISA Kit;Human jumonji domain containing 2 ELISA Kit;Human jumonji domain containing 2A ELISA Kit;Human lysine (K)-specific demethylase 4A ELISA Kit;Human tudor domain containing 14A ELISA Kit;
Catalog
E023202
species
Human
GeneKDM4A
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lysine Specific Demethylase 4A (KDM4A). No significant cross-reactivity or interference between Lysine Specific Demethylase 4A (KDM4A) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.126ng/mL.
Intended UseHuman KDM4A ELISA Kit allows for the in vitro quantitative determination of KDM4A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysine Specific Demethylase 4A (KDM4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysine Specific Demethylase 4A (KDM4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysine Specific Demethylase 4A (KDM4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysine Specific Demethylase 4A (KDM4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Lysine-specific demethylase 4A Protein information
Uniprot ID KDM4A_HUMAN
Uniprot AC O75164; Q5VVB1;
UniGene Hs.155983;
GeneID 9682
KEGG hsa:9682;
Human Lysine-specific demethylase 4A Protein SEQUENCE
SEQUENCE 1064 AA; 120662 MW; 4A811BEECFEDC6B3 CRC64;
MASESETLNP SARIMTFYPT MEEFRNFSRY IAYIESQGAH RAGLAKVVPP
KEWKPRASYD DIDDLVIPAP IQQLVTGQSG LFTQYNIQKK AMTVREFRKI
ANSDKYCTPR YSEFEELERK YWKNLTFNPP IYGADVNGTL YEKHVDEWNI
GRLRTILDLV EKESGITIEG VNTPYLYFGM WKTSFAWHTE DMDLYSINYL
HFGEPKSWYS VPPEHGKRLE RLAKGFFPGS AQSCEAFLRH KMTLISPLML
KKYGIPFDKV TQEAGEFMIT FPYGYHAGFN HGFNCAESTN FATRRWIEYG
KQAVLCSCRK DMVKISMDVF VRKFQPERYK LWKAGKDNTV IDHTLPTPEA
AEFLKESELP PRAGNEEECP EEDMEGVEDG EEGDLKTSLA KHRIGTKRHR
VCLEIPQEVS QSELFPKEDL SSEQYEMTEC PAALAPVRPT HSSVRQVEDG
LTFPDYSDST EVKFEELKNV KLEEEDEEEE QAAAALDLSV NPASVGGRLV
FSGSKKKSSS SLGSGSSRDS ISSDSETSEP LSCRAQGQTG VLTVHSYAKG
DGRVTVGEPC TRKKGSAARS FSERELAEVA DEYMFSLEEN KKSKGRRQPL
SKLPRHHPLV LQECVSDDET SEQLTPEEEA EETEAWAKPL SQLWQNRPPN
FEAEKEFNET MAQQAPHCAV CMIFQTYHQV EFGGFNQNCG NASDLAPQKQ
RTKPLIPEMC FTSTGCSTDI NLSTPYLEED GTSILVSCKK CSVRVHASCY
GVPPAKASED WMCSRCSANA LEEDCCLCSL RGGALQRAND DRWVHVSCAV
AILEARFVNI AERSPVDVSK IPLPRFKLKC IFCKKRRKRT AGCCVQCSHG
RCPTAFHVSC AQAAGVMMQP DDWPFVVFIT CFRHKIPNLE RAKGALQSIT
AGQKVISKHK NGRFYQCEVV RLTTETFYEV NFDDGSFSDN LYPEDIVSQD
CLQFGPPAEG EVVQVRWTDG QVYGAKFVAS HPIQMYQVEF EDGSQLVVKR
DDVYTLDEEL PKRVKSRLSV ASDMRFNEIF TEKEVKQEKK RQRVINSRYR
EDYIEPALYR AIME
UCSC uc001cjx.4; human. [O75164-1];

Category

Human KDM4A ELISA Kit