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Human MTOR ELISA Kit

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  • Alternative name

    Human FK506-binding protein 12-rapamycin complex-associated protein 1 ELISA Kit;Human FKBP12-rapamycin complex-associated protein ELISA Kit;Human mammalian target of rapamycin ELISA Kit;Human mTOR ELISA Kit;Human mechanistic target of rapamycin ELISA Kit;Human rapamycin and FKBP12 target 1 ELISA Kit;Human rapamycin target protein 1 ELISA Kit;Human FRAP ELISA Kit;Human FRAP1 ELISA Kit;Human FRAP2 ELISA Kit;Human RAFT1 ELISA Kit;Human RAPT1 ELISA Kit;Human SKS ELISA Kit;Human serine/threonine-protein kinase mTOR ELISA Kit;Human FK506 binding protein 12-rapamycin associated protein 2 ELISA Kit;Human FKBP-rapamycin associated protein ELISA Kit;Human FKBP12-rapamycin complex-associated protein 1 ELISA Kit;Human mechanistic target of rapamycin (serine/threonine kinase) ELISA Kit;Human rapamycin associated protein FRAP2 ELISA Kit;
  • Catalog
    E023569
  • species
    Human
  • GeneMTOR
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human mTOR. No significant cross-reactivity or interference between human mTOR and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human mTOR is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman MTOR ELISA Kit allows for the in vitro quantitative determination of MTOR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for mTOR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any mTOR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mTOR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mTOR bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Serine/threonine-protein kinase mTOR Protein information
  • Uniprot ID MTOR_HUMAN
  • Uniprot AC P42345; Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3;
  • UniGene Hs.338207;
  • GeneID 2475
  • KEGG hsa:2475;
  • Human Serine/threonine-protein kinase mTOR Protein SEQUENCE

  • SEQUENCE 2549 AA; 288892 MW; 7D9AD6E784882AB4 CRC64;

    MLGTGPAAAT TAATTSSNVS VLQQFASGLK SRNEETRAKA AKELQHYVTM

    ELREMSQEES TRFYDQLNHH IFELVSSSDA NERKGGILAI ASLIGVEGGN

    ATRIGRFANY LRNLLPSNDP VVMEMASKAI GRLAMAGDTF TAEYVEFEVK

    RALEWLGADR NEGRRHAAVL VLRELAISVP TFFFQQVQPF FDNIFVAVWD

    PKQAIREGAV AALRACLILT TQREPKEMQK PQWYRHTFEE AEKGFDETLA

    KEKGMNRDDR IHGALLILNE LVRISSMEGE RLREEMEEIT QQQLVHDKYC

    KDLMGFGTKP RHITPFTSFQ AVQPQQSNAL VGLLGYSSHQ GLMGFGTSPS

    PAKSTLVESR CCRDLMEEKF DQVCQWVLKC RNSKNSLIQM TILNLLPRLA

    AFRPSAFTDT QYLQDTMNHV LSCVKKEKER TAAFQALGLL SVAVRSEFKV

    YLPRVLDIIR AALPPKDFAH KRQKAMQVDA TVFTCISMLA RAMGPGIQQD

    IKELLEPMLA VGLSPALTAV LYDLSRQIPQ LKKDIQDGLL KMLSLVLMHK

    PLRHPGMPKG LAHQLASPGL TTLPEASDVG SITLALRTLG SFEFEGHSLT

    QFVRHCADHF LNSEHKEIRM EAARTCSRLL TPSIHLISGH AHVVSQTAVQ

    VVADVLSKLL VVGITDPDPD IRYCVLASLD ERFDAHLAQA ENLQALFVAL

    NDQVFEIREL AICTVGRLSS MNPAFVMPFL RKMLIQILTE LEHSGIGRIK

    EQSARMLGHL VSNAPRLIRP YMEPILKALI LKLKDPDPDP NPGVINNVLA

    TIGELAQVSG LEMRKWVDEL FIIIMDMLQD SSLLAKRQVA LWTLGQLVAS

    TGYVVEPYRK YPTLLEVLLN FLKTEQNQGT RREAIRVLGL LGALDPYKHK

    VNIGMIDQSR DASAVSLSES KSSQDSSDYS TSEMLVNMGN LPLDEFYPAV

    SMVALMRIFR DQSLSHHHTM VVQAITFIFK SLGLKCVQFL PQVMPTFLNV

    IRVCDGAIRE FLFQQLGMLV SFVKSHIRPY MDEIVTLMRE FWVMNTSIQS

    TIILLIEQIV VALGGEFKLY LPQLIPHMLR VFMHDNSPGR IVSIKLLAAI

    QLFGANLDDY LHLLLPPIVK LFDAPEAPLP SRKAALETVD RLTESLDFTD

    YASRIIHPIV RTLDQSPELR STAMDTLSSL VFQLGKKYQI FIPMVNKVLV

    RHRINHQRYD VLICRIVKGY TLADEEEDPL IYQHRMLRSG QGDALASGPV

    ETGPMKKLHV STINLQKAWG AARRVSKDDW LEWLRRLSLE LLKDSSSPSL

    RSCWALAQAY NPMARDLFNA AFVSCWSELN EDQQDELIRS IELALTSQDI

    AEVTQTLLNL AEFMEHSDKG PLPLRDDNGI VLLGERAAKC RAYAKALHYK

    ELEFQKGPTP AILESLISIN NKLQQPEAAA GVLEYAMKHF GELEIQATWY

    EKLHEWEDAL VAYDKKMDTN KDDPELMLGR MRCLEALGEW GQLHQQCCEK

    WTLVNDETQA KMARMAAAAA WGLGQWDSME EYTCMIPRDT HDGAFYRAVL

    ALHQDLFSLA QQCIDKARDL LDAELTAMAG ESYSRAYGAM VSCHMLSELE

    EVIQYKLVPE RREIIRQIWW ERLQGCQRIV EDWQKILMVR SLVVSPHEDM

    RTWLKYASLC GKSGRLALAH KTLVLLLGVD PSRQLDHPLP TVHPQVTYAY

    MKNMWKSARK IDAFQHMQHF VQTMQQQAQH AIATEDQQHK QELHKLMARC

    FLKLGEWQLN LQGINESTIP KVLQYYSAAT EHDRSWYKAW HAWAVMNFEA

    VLHYKHQNQA RDEKKKLRHA SGANITNATT AATTAATATT TASTEGSNSE

    SEAESTENSP TPSPLQKKVT EDLSKTLLMY TVPAVQGFFR SISLSRGNNL

    QDTLRVLTLW FDYGHWPDVN EALVEGVKAI QIDTWLQVIP QLIARIDTPR

    PLVGRLIHQL LTDIGRYHPQ ALIYPLTVAS KSTTTARHNA ANKILKNMCE

    HSNTLVQQAM MVSEELIRVA ILWHEMWHEG LEEASRLYFG ERNVKGMFEV

    LEPLHAMMER GPQTLKETSF NQAYGRDLME AQEWCRKYMK SGNVKDLTQA

    WDLYYHVFRR ISKQLPQLTS LELQYVSPKL LMCRDLELAV PGTYDPNQPI

    IRIQSIAPSL QVITSKQRPR KLTLMGSNGH EFVFLLKGHE DLRQDERVMQ

    LFGLVNTLLA NDPTSLRKNL SIQRYAVIPL STNSGLIGWV PHCDTLHALI

    RDYREKKKIL LNIEHRIMLR MAPDYDHLTL MQKVEVFEHA VNNTAGDDLA

    KLLWLKSPSS EVWFDRRTNY TRSLAVMSMV GYILGLGDRH PSNLMLDRLS

    GKILHIDFGD CFEVAMTREK FPEKIPFRLT RMLTNAMEVT GLDGNYRITC

    HTVMEVLREH KDSVMAVLEA FVYDPLLNWR LMDTNTKGNK RSRTRTDSYS

    AGQSVEILDG VELGEPAHKK TGTTVPESIH SFIGDGLVKP EALNKKAIQI

    INRVRDKLTG RDFSHDDTLD VPTQVELLIK QATSHENLCQ CYIGWCPFW

  • UCSC uc001asd.4; human.;



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