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Human MAN1A1 ELISA Kit

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  • Alternative name

    Human man(9)-alpha-mannosidase ELISA Kit;Human Man9-mannosidase ELISA Kit;Human mannosidase alpha class 1A member 1 ELISA Kit;Human processing alpha-1,2-mannosidase IA ELISA Kit;Human alpha-1,2-mannosidase IA ELISA Kit;Human HUMM3 ELISA Kit;Human HUMM9 ELISA Kit;Human MAN9 ELISA Kit;Human mannosyl-oligosaccharide 1,2-alpha-mannosidase IA ELISA Kit;
  • Catalog
    E023625
  • species
    Human
  • GeneMAN1A1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mannosidase Alpha Class 1A Member 1 (MAN1A1). No significant cross-reactivity or interference between Mannosidase Alpha Class 1A Member 1 (MAN1A1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman MAN1A1 ELISA Kit allows for the in vitro quantitative determination of MAN1A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 1A Member 1 (MAN1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 1A Member 1 (MAN1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 1A Member 1 (MAN1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosidase Alpha Class 1A Member 1 (MAN1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA Protein information
  • Uniprot ID MA1A1_HUMAN
  • Uniprot AC P33908; E7EU32; Q6P052; Q9NU44; Q9UJI3;
  • UniGene Hs.102788; Hs.742378;
  • GeneID 4121
  • KEGG hsa:4121;
  • Human Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA Protein SEQUENCE

  • SEQUENCE 653 AA; 72969 MW; 9B164926636D8554 CRC64;

    MPVGGLLPLF SSPAGGVLGG GLGGGGGRKG SGPAALRLTE KFVLLLVFSA

    FITLCFGAIF FLPDSSKLLS GVLFHSSPAL QPAADHKPGP GARAEDAAEG

    RARRREEGAP GDPEAALEDN LARIRENHER ALREAKETLQ KLPEEIQRDI

    LLEKKKVAQD QLRDKAPFRG LPPVDFVPPI GVESREPADA AIREKRAKIK

    EMMKHAWNNY KGYAWGLNEL KPISKGGHSS SLFGNIKGAT IVDALDTLFI

    MEMKHEFEEA KSWVEENLDF NVNAEISVFE VNIRFVGGLL SAYYLSGEEI

    FRKKAVELGV KLLPAFHTPS GIPWALLNMK SGIGRNWPWA SGGSSILAEF

    GTLHLEFMHL SHLSGNPIFA EKVMNIRTVL NKLEKPQGLY PNYLNPSSGQ

    WGQHHVSVGG LGDSFYEYLL KAWLMSDKTD LEAKKMYFDA VQAIETHLIR

    KSSSGLTYIA EWKGGLLEHK MGHLTCFAGG MFALGADAAP EGMAQHYLEL

    GAEIARTCHE SYNRTFMKLG PEAFRFDGGV EAIATRQNEK YYILRPEVME

    TYMYMWRLTH DPKYRKWAWE AVEALENHCR VNGGYSGLRD VYLLHESYDD

    VQQSFFLAET LKYLYLIFSD DDLLPLEHWI FNSEAHLLPI LPKDKKEVEI

    REE

  • UCSC uc003pym.3; human. [P33908-1];



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