Human PRSS27 ELISA Kit

Alternative name
Human marapsin ELISA Kit;Human pancreasin ELISA Kit;Human MPN ELISA Kit;Human CAPH2 ELISA Kit;Human protease, serine 27 ELISA Kit;Human serine protease 27 ELISA Kit;Human channel-activating protease 2 ELISA Kit;
Catalog
E023663
species
Human
GenePRSS27
SpecificityNatural and recombinant Human Marapsin/Pancresin
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
Intended UseHuman PRSS27 ELISA Kit allows for the in vitro quantitative determination of PRSS27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Human Marapsin/Pancresin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Marapsin/Pancresin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: M35-K290) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Marapsin/Pancresin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Marapsin/Pancresin amount of sample captured in plate. Background/Introduction: Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.
Human Serine protease 27 Protein information
Uniprot ID PRS27_HUMAN
Uniprot AC Q9BQR3;
UniGene Hs.332878;
GeneID 83886
KEGG hsa:83886;
Human Serine protease 27 Protein SEQUENCE
SEQUENCE 290 AA; 31940 MW; 67BDC93EC70BFF7B CRC64;
MRRPAAVPLL LLLCFGSQRA KAATACGRPR MLNRMVGGQD TQEGEWPWQV
SIQRNGSHFC GGSLIAEQWV LTAAHCFRNT SETSLYQVLL GARQLVQPGP
HAMYARVRQV ESNPLYQGTA SSADVALVEL EAPVPFTNYI LPVCLPDPSV
IFETGMNCWV TGWGSPSEED LLPEPRILQK LAVPIIDTPK CNLLYSKDTE
FGYQPKTIKN DMLCAGFEEG KKDACKGDSG GPLVCLVGQS WLQAGVISWG
EGCARQNRPG VYIRVTAHHN WIHRIIPKLQ FQPARLGGQK
UCSC uc002crf.4; human.;

Category

Human PRSS27 ELISA Kit

Human marapsin ELISA Kit;Human pancreasin ELISA Kit;Human MPN ELISA Kit;Human CAPH2 ELISA Kit;Human protease, serine 27 ELISA Kit;Human serine protease 27 ELISA Kit;Human channel-activating protease 2 ELISA Kit;