Human MMP1 ELISA Kit

Alternative name
Human fibroblast collagenase ELISA Kit;Human Matrix metalloproteinase-1 ELISA Kit;Human MMP-1 ELISA Kit;Human CLG ELISA Kit;Human CLGN ELISA Kit;Human matrix metallopeptidase 1 ELISA Kit;Human interstitial collagenase ELISA Kit;Human matrix metallopeptidase 1 (interstitial collagenase) ELISA Kit;Human matrix metalloprotease 1 ELISA Kit;Human matrix metalloproteinase 1 ELISA Kit;
Catalog
E023734
species
Human
GeneMMP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MMP1. No significant cross-reactivity or interference between Human MMP1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.078 ng/ml
Intended UseHuman MMP1 ELISA Kit allows for the in vitro quantitative determination of MMP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 27 kDa interstitial collagenase Protein information
Uniprot ID MMP1_HUMAN
Uniprot AC P03956; P08156;
UniGene Hs.83169;
GeneID 4312
KEGG hsa:4312;
Human 27 kDa interstitial collagenase Protein SEQUENCE
SEQUENCE 469 AA; 54007 MW; 4B1361DCF4C54B20 CRC64;
MHSFPPLLLL LFWGVVSHSF PATLETQEQD VDLVQKYLEK YYNLKNDGRQ
VEKRRNSGPV VEKLKQMQEF FGLKVTGKPD AETLKVMKQP RCGVPDVAQF
VLTEGNPRWE QTHLTYRIEN YTPDLPRADV DHAIEKAFQL WSNVTPLTFT
KVSEGQADIM ISFVRGDHRD NSPFDGPGGN LAHAFQPGPG IGGDAHFDED
ERWTNNFREY NLHRVAAHEL GHSLGLSHST DIGALMYPSY TFSGDVQLAQ
DDIDGIQAIY GRSQNPVQPI GPQTPKACDS KLTFDAITTI RGEVMFFKDR
FYMRTNPFYP EVELNFISVF WPQLPNGLEA AYEFADRDEV RFFKGNKYWA
VQGQNVLHGY PKDIYSSFGF PRTVKHIDAA LSEENTGKTY FFVANKYWRY
DEYKRSMDPG YPKMIAHDFP GIGHKVDAVF MKDGFFYFFH GTRQYKFDPK
TKRILTLQKA NSWFNCRKN
UCSC uc001phi.3; human.;

Category

Human MMP1 ELISA Kit