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Human MMP12 ELISA Kit

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  • Alternative name

    Human MME ELISA Kit;Human Macrophage elastase ELISA Kit;Human ME ELISA Kit;Human HME ELISA Kit;Human Matrix metalloproteinase-12 ELISA Kit;Human MMP-12 ELISA Kit;Human matrix metallopeptidase 12 ELISA Kit;Human macrophage metalloelastase ELISA Kit;Human matrix metallopeptidase 12 (macrophage elastase) ELISA Kit;Human matrix metalloproteinase 12 (macrophage elastase) ELISA Kit;
  • Catalog
    E023737
  • species
    Human
  • GeneMMP12
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human MMP-12. No significant cross-reactivity or interference between human MMP-12 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human MMP-12 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
  • Intended UseHuman MMP12 ELISA Kit allows for the in vitro quantitative determination of MMP12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Macrophage metalloelastase Protein information
  • Uniprot ID MMP12_HUMAN
  • Uniprot AC P39900; B2R9X8; B7ZLF6; Q2M1L9;
  • UniGene Hs.1695; Hs.709832;
  • GeneID 4321
  • KEGG hsa:4321;
  • Human Macrophage metalloelastase Protein SEQUENCE

  • SEQUENCE 470 AA; 54002 MW; 8C745EEA8C0EA216 CRC64;

    MKFLLILLLQ ATASGALPLN SSTSLEKNNV LFGERYLEKF YGLEINKLPV

    TKMKYSGNLM KEKIQEMQHF LGLKVTGQLD TSTLEMMHAP RCGVPDVHHF

    REMPGGPVWR KHYITYRINN YTPDMNREDV DYAIRKAFQV WSNVTPLKFS

    KINTGMADIL VVFARGAHGD FHAFDGKGGI LAHAFGPGSG IGGDAHFDED

    EFWTTHSGGT NLFLTAVHEI GHSLGLGHSS DPKAVMFPTY KYVDINTFRL

    SADDIRGIQS LYGDPKENQR LPNPDNSEPA LCDPNLSFDA VTTVGNKIFF

    FKDRFFWLKV SERPKTSVNL ISSLWPTLPS GIEAAYEIEA RNQVFLFKDD

    KYWLISNLRP EPNYPKSIHS FGFPNFVKKI DAAVFNPRFY RTYFFVDNQY

    WRYDERRQMM DPGYPKLITK NFQGIGPKID AVFYSKNKYY YFFQGSNQFE

    YDFLLQRITK TLKSNSWFGC

  • UCSC uc031yde.2; human.;



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