Human MMP8 ELISA Kit

Alternative name
Human matrix metalloproteinase-8 ELISA Kit;Human MMP-8 ELISA Kit;Human PMNL collagenase ELISA Kit;Human PMNL-CL ELISA Kit;Human CLG1 ELISA Kit;Human HNC ELISA Kit;Human matrix metallopeptidase 8 ELISA Kit;Human neutrophil collagenase ELISA Kit;Human PMN leukocyte collagenase ELISA Kit;Human collagenase 2 ELISA Kit;Human matrix metalloproteinase 8 (neutrophil collagenase) ELISA Kit;
Catalog
E023753
species
Human
GeneMMP8
SpecificityThis assay has high sensitivity and excellent specificity for detection of human MMP-8. No significant cross-reactivity or interference between human MMP-8 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human MMP-8 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman MMP8 ELISA Kit allows for the in vitro quantitative determination of MMP8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Neutrophil collagenase Protein information
Uniprot ID MMP8_HUMAN
Uniprot AC P22894; Q45F99;
UniGene Hs.161839;
GeneID 4317
KEGG hsa:4317;
Human Neutrophil collagenase Protein SEQUENCE
SEQUENCE 467 AA; 53412 MW; 4D4602A53AD7F8BC CRC64;
MFSLKTLPFL LLLHVQISKA FPVSSKEKNT KTVQDYLEKF YQLPSNQYQS
TRKNGTNVIV EKLKEMQRFF GLNVTGKPNE ETLDMMKKPR CGVPDSGGFM
LTPGNPKWER TNLTYRIRNY TPQLSEAEVE RAIKDAFELW SVASPLIFTR
ISQGEADINI AFYQRDHGDN SPFDGPNGIL AHAFQPGQGI GGDAHFDAEE
TWTNTSANYN LFLVAAHEFG HSLGLAHSSD PGALMYPNYA FRETSNYSLP
QDDIDGIQAI YGLSSNPIQP TGPSTPKPCD PSLTFDAITT LRGEILFFKD
RYFWRRHPQL QRVEMNFISL FWPSLPTGIQ AAYEDFDRDL IFLFKGNQYW
ALSGYDILQG YPKDISNYGF PSSVQAIDAA VFYRSKTYFF VNDQFWRYDN
QRQFMEPGYP KSISGAFPGI ESKVDAVFQQ EHFFHVFSGP RYYAFDLIAQ
RVTRVARGNK WLNCRYG
UCSC uc001phe.2; human.;

Category

Human MMP8 ELISA Kit