Human MBD2 ELISA Kit

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  • Alternative name

    Human demethylase ELISA Kit;Human DMTase ELISA Kit;Human Methyl-CpG-binding protein MBD2 ELISA Kit;Human NY-CO-41 ELISA Kit;Human methyl-CpG binding domain protein 2 ELISA Kit;Human methyl-CpG-binding domain protein 2 ELISA Kit;

  • Catalog
    E023856
  • species
    Human
  • GeneMBD2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MBD2. No significant cross-reactivity or interference between Human MBD2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human MBD2 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseHuman MBD2 ELISA Kit allows for the in vitro quantitative determination of MBD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MBD2 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MBD2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human MBD2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MBD2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MBD2. You can calculate the concentration of Human MBD2 in the samples by comparing the OD of the samples to the standard curve.
  • Human Methyl-CpG-binding domain protein 2 Protein information
  • Uniprot ID MBD2_HUMAN
  • Uniprot AC Q9UBB5; O95242; Q9UIS8;
  • UniGene Hs.25674;
  • GeneID 8932
  • KEGG hsa:8932;
  • Human Methyl-CpG-binding domain protein 2 Protein SEQUENCE
  • SEQUENCE 411 AA; 43255 MW; FC4E5E0CF9BA0FFA CRC64;

    MRAHPGGGRC CPEQEEGESA AGGSGAGGDS AIEQGGQGSA LAPSPVSGVR

    REGARGGGRG RGRWKQAGRG GGVCGRGRGR GRGRGRGRGR GRGRGRPPSG

    GSGLGGDGGG CGGGGSGGGG APRREPVPFP SGSAGPGPRG PRATESGKRM

    DCPALPPGWK KEEVIRKSGL SAGKSDVYYF SPSGKKFRSK PQLARYLGNT

    VDLSSFDFRT GKMMPSKLQK NKQRLRNDPL NQNKGKPDLN TTLPIRQTAS

    IFKQPVTKVT NHPSNKVKSD PQRMNEQPRQ LFWEKRLQGL SASDVTEQII

    KTMELPKGLQ GVGPGSNDET LLSAVASALH TSSAPITGQV SAAVEKNPAV

    WLNTSQPLCK AFIVTDEDIR KQEERVQQVR KKLEEALMAD ILSRAADTEE

    MDIEMDSGDE A

  • UCSC uc002lfg.2; human. [Q9UBB5-1];



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