Human MDM2 ELISA Kit

Alternative name
Human Double minute 2 protein ELISA Kit;Human hdm2 ELISA Kit;Human oncoprotein Mdm2 ELISA Kit;Human RING-type E3 ubiquitin transferase Mdm2 ELISA Kit;Human p53-binding protein Mdm2 ELISA Kit;Human ACTFS ELISA Kit;Human HDMX ELISA Kit;Human MDM2 proto-oncogene ELISA Kit;Human E3 ubiquitin-protein ligase Mdm2 ELISA Kit;Human MDM2 oncogene, E3 ubiquitin protein ligase ELISA Kit;Human MDM2 proto-oncogene, E3 ubiquitin protein ligase ELISA Kit;Human Mdm2, p53 E3 ubiquitin protein ligase homolog ELISA Kit;Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein ELISA Kit;Human double minute 2, human homolog of; p53-binding protein ELISA Kit;
Catalog
E023891
species
Human
GeneMDM2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mdm2 p53 Binding Protein Homolog (MDM2). No significant cross-reactivity or interference between Mdm2 p53 Binding Protein Homolog (MDM2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman MDM2 ELISA Kit allows for the in vitro quantitative determination of MDM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mdm2 p53 Binding Protein Homolog (MDM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mdm2 p53 Binding Protein Homolog (MDM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mdm2 p53 Binding Protein Homolog (MDM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mdm2 p53 Binding Protein Homolog (MDM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human E3 ubiquitin-protein ligase Mdm2 Protein information
Uniprot ID MDM2_HUMAN
Uniprot AC Q13301; Q53XW0; Q71TW9; Q8WYJ1; Q8WYJ2; Q9UGI3; Q9UMT8;
UniGene Hs.484551; Hs.733536;
GeneID 4193
KEGG hsa:4193;
Human E3 ubiquitin-protein ligase Mdm2 Protein SEQUENCE
SEQUENCE 491 AA; 55233 MW; F37CE163876BC983 CRC64;
MCNTNMSVPT DGAVTTSQIP ASEQETLVRP KPLLLKLLKS VGAQKDTYTM
KEVLFYLGQY IMTKRLYDEK QQHIVYCSND LLGDLFGVPS FSVKEHRKIY
TMIYRNLVVV NQQESSDSGT SVSENRCHLE GGSDQKDLVQ ELQEEKPSSS
HLVSRPSTSS RRRAISETEE NSDELSGERQ RKRHKSDSIS LSFDESLALC
VIREICCERS SSSESTGTPS NPDLDAGVSE HSGDWLDQDS VSDQFSVEFE
VESLDSEDYS LSEEGQELSD EDDEVYQVTV YQAGESDTDS FEEDPEISLA
DYWKCTSCNE MNPPLPSHCN RCWALRENWL PEDKGKDKGE ISEKAKLENS
TQAEEGFDVP DCKKTIVNDS RESCVEENDD KITQASQSQE SEDYSQPSTS
SSIIYSSQED VKEFEREETQ DKEESVESSL PLNAIEPCVI CQGRPKNGCI
VHGKTGHLMA CFTCAKKLKK RNKPCPVCRQ PIQMIVLTYF P
UCSC uc001sui.6; human. [Q00987-1];

Category

Human MDM2 ELISA Kit