Human MTBP ELISA Kit

Alternative name
Human hMTBP ELISA Kit;Human MDM2BP ELISA Kit;Human MDM2 binding protein ELISA Kit;Human mdm2-binding protein ELISA Kit;Human MDM2 (mouse double minute 2)-binding protein, 104kD ELISA Kit;Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein, 104kDa ELISA Kit;
Catalog
E023894
species
Human
GeneMTBP
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman MTBP ELISA Kit allows for the in vitro quantitative determination of MTBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MTBP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MTBP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MTBP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MTBP can be calculated.
Human Mdm2-binding protein Protein information
Uniprot ID MTBP_HUMAN
Uniprot AC Q96DY7; B4DUR5; Q9HA89;
UniGene Hs.657656;
GeneID 27085
KEGG hsa:27085;
Human Mdm2-binding protein Protein SEQUENCE
SEQUENCE 904 AA; 102193 MW; 753DF4A98AB62E4F CRC64;
MDRYLLLVIW GEGKFPSAAS REAEHGPEVS SGEGTENQPD FTAANVYHLL
KRSISASINP EDSTFPACSV GGIPGSKKWF FAVQAIYGFY QFCSSDWQEI
HFDTEKDKIE DVLQTNIEEC LGAVECFEEE DSNSRESLSL ADLYEEAAEN
LHQLSDKLPA PGRAMVDIIL LLSDKDPPKL KDYLPTVGAL KHLREWYSAK
ITIAGNHCEI NCQKIAEYLS ANVVSLEDLR NVIDSKELWR GKIQIWERKF
GFEISFPEFC LKGVTLKNFS TSNLNTDFLA KKIIPSKDKN ILPKVFHYYG
PALEFVQMIK LSDLPSCYMS DIEFELGLTN STKQNSVLLL EQISSLCSKV
GALFVLPCTI SNILIPPPNQ LSSRKWKEYI AKKPKTISVP DVEVKGECSS
YYLLLQGNGN RRCKATLIHS ANQINGSFAL NLIHGKMKTK TEEAKLSFPF
DLLSLPHFSG EQIVQREKQL ANVQVLALEE CLKRRKLAKQ PETVSVAELK
SLLVLTRKHF LDYFDAVIPK MILRKMDKIK TFNILNDFSP VEPNSSSLME
TNPLEWPERH VLQNLETFEK TKQKMRTGSL PHSSEQLLGH KEGPRDSITL
LDAKELLKYF TSDGLPIGDL QPLPIQKGEK TFVLTPELSP GKLQVLPFEK
ASVCHYHGIE YCLDDRKALE RDGGFSELQS RLIRYETQTT CTRESFPVPT
VLSPLPSPVV SSDPGSVPDG EVLQNELRTE VSRLKRRSKD LNCLYPRKRL
VKSESSESLL SQTTGNSNHY HHHVTSRKPQ TERSLPVTCP LVPIPSCETP
KLATKTSSGQ KSMHESKTSR QIKESRSQKH TRILKEVVTE TLKKHSITET
HECFTACSQR LFEISKFYLK DLKTSRGLFE EMKKTANNNA VQVIDWVLEK
TSKK
UCSC uc003ypc.3; human. [Q96DY7-1];

Category

Human MTBP ELISA Kit

Human hMTBP ELISA Kit;Human MDM2BP ELISA Kit;Human MDM2 binding protein ELISA Kit;Human mdm2-binding protein ELISA Kit;Human MDM2 (mouse double minute 2)-binding protein, 104kD ELISA Kit;Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein, 104kDa ELISA Kit;