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Human MC1R ELISA Kit

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  • Alternative name

    Human MSH-R ELISA Kit;Human Melanocortin receptor 1 ELISA Kit;Human MC1-R ELISA Kit;Human MSHR ELISA Kit;Human CMM5 ELISA Kit;Human SHEP2 ELISA Kit;Human melanocortin 1 receptor ELISA Kit;Human melanocyte-stimulating hormone receptor ELISA Kit;Human alpha melanocyte stimulating hormone receptor ELISA Kit;Human melanotropin receptor ELISA Kit;
  • Catalog
    E024002
  • species
    Human
  • GeneMC1R
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MC1R. No significant cross-reactivity or interference between MC1R and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MC1R and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MC1R ELISA Kit allows for the in vitro quantitative determination of MC1R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses||This MC1R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MC1R. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: MC1R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MC1R antibody and an MC1R-HRP conjugate. The assay sample and buffer are incubated together with MC1R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MC1R concentration since MC1R from samples and MC1R-HRP conjugate compete for the anti-MC1R antibody binding site. Since the number of sites is limited, as more sites are occupied by MC1R from the sample, fewer sites are left to bind MC1R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MC1R concentration in each sample is interpolated from this standard curve.
  • Human Melanocyte-stimulating hormone receptor Protein information
  • Uniprot ID MSHR_HUMAN
  • Uniprot AC Q9UN58; Q9UN59; Q9UN60; Q9UN61; Q9UN62;
  • UniGene Hs.513829; Hs.734194;
  • GeneID 4157
  • KEGG hsa:4157;
  • Human Melanocyte-stimulating hormone receptor Protein SEQUENCE

  • SEQUENCE 317 AA; 34706 MW; CB67405A562C29B2 CRC64;

    MAVQGSQRRL LGSLNSTPTA IPQLGLAANQ TGARCLEVSI SDGLFLSLGL

    VSLVENALVV ATIAKNRNLH SPMYCFICCL ALSDLLVSGS NVLETAVILL

    LEAGALVARA AVLQQLDNVI DVITCSSMLS SLCFLGAIAV DRYISIFYAL

    RYHSIVTLPR ARRAVAAIWV ASVVFSTLFI AYYDHVAVLL CLVVFFLAML

    VLMAVLYVHM LARACQHAQG IARLHKRQRP VHQGFGLKGA VTLTILLGIF

    FLCWGPFFLH LTLIVLCPEH PTCGCIFKNF NLFLALIICN AIIDPLIYAF

    HSQELRRTLK EVLTCSW




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