Human MAGED4B ELISA Kit

Alternative name
Human MAGE-D4 antigen ELISA Kit;Human MAGE-E1 antigen ELISA Kit;Human MAGE family member D4B ELISA Kit;Human melanoma-associated antigen D4 ELISA Kit;Human melanoma antigen family D, 4B ELISA Kit;Human melanoma antigen family D4B ELISA Kit;
Catalog
E024028
species
Human
GeneMAGED4B
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MAGED4B. No significant cross-reactivity or interference between Human MAGED4B and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman MAGED4B ELISA Kit allows for the in vitro quantitative determination of MAGED4B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAGED4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MAGED4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MAGED4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MAGED4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Melanoma-associated antigen D4 Protein information
Uniprot ID MAGD4_HUMAN
Uniprot AC Q9H217;
UniGene Hs.522650; Hs.571729; Hs.733143;
GeneID 81557
KEGG hsa:81557;
Human Melanoma-associated antigen D4 Protein SEQUENCE
SEQUENCE 741 AA; 81378 MW; 56B073324C12A8EC CRC64;
MAEGSFSVQS ESYSVEDMDE GSDEVGEEEM VEGNDYEEFG AFGGYGTLTS
FDIHILRAFG SLGPGLRILS NEPWELENPV LAQTLVEALQ LDPETLANET
AARAANVARA AASNRAARAA AAAARTAFSQ VVASHRVATP QVSGEDTQPT
TYAAEAQGPT PEPPLASPQT SQMLVTSKMA APEAPATSAQ SQTGSPAQEA
ATEGPSSACA FSQAPCAREV DANRPSTAFL GQNDVFDFTQ PAGVSGMAFP
RPKRPAPAQE AATEGPSAAS GVPQTGPGRE VAATRPKTTK SGKALAKTRW
VEPQNVVAAA AAKAKMATSI PEPEGAAAAT AQHSAEPWAR MGGKRTKKSK
HLDDEYESSE EERETPAVPP TWRASQPSLT VRAQLAPRPP MAPRSQIPSR
HVLCLPPRNV TLLQERANKL VKYLMIKDYK KIPIKRADML KDVIREYDEH
FPEIIERATY TLEKKFGIHL KEIDKEEHLY ILVCTRDSSA RLLGKTKDTP
RLSLLLVILG VIFMNGNRAS EAVLWEALRK MGLRPGVRHP FLGDLRKLIT
DDFVKQKYLE YKKIPNSNPP EYEFLWGLRA RHETSKMRVL RFIAQNQNRD
PREWKAHFLE AVDDAFKTMD VDMAEEHARA QMRAQMNIGD EALIGRWSWD
DIQVELLTWD EDGDFGDAWA RIPFAFWARY HQYILNSNRA NRRATWRAGV
SSGTNGGAST SVLDGPSTSS TIRTRNAARA GASFFSWIQH R
UCSC uc004dpr.5; human. [Q96JG8-1];

Category

Human MAGED4B ELISA Kit

Human MAGE-D4 antigen ELISA Kit;Human MAGE-E1 antigen ELISA Kit;Human MAGE family member D4B ELISA Kit;Human melanoma-associated antigen D4 ELISA Kit;Human melanoma antigen family D, 4B ELISA Kit;Human melanoma antigen family D4B ELISA Kit;