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Human MEP1B ELISA Kit

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  • Alternative name

    Human endopeptidase-2 ELISA Kit;Human meprin B ELISA Kit;Human N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta ELISA Kit;Human PABA peptide hydrolase ELISA Kit;Human PPH beta ELISA Kit;Human meprin A subunit beta ELISA Kit;Human N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit ELISA Kit;Human meprin A, beta ELISA Kit;
  • Catalog
    E024171
  • species
    Human
  • GeneMEP1B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Meprin A Beta (MEP1b). No significant cross-reactivity or interference between Meprin A Beta (MEP1b) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.117ng/mL.
  • Intended UseHuman MEP1B ELISA Kit allows for the in vitro quantitative determination of MEP1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meprin A Beta (MEP1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meprin A Beta (MEP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meprin A Beta (MEP1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Meprin A Beta (MEP1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Meprin A subunit beta Protein information
  • Uniprot ID MEP1B_HUMAN
  • Uniprot AC Q16820; B7ZM35; B9EGL6; Q670J1;
  • UniGene Hs.194777;
  • GeneID 4225
  • KEGG hsa:4225;
  • Human Meprin A subunit beta Protein SEQUENCE

  • SEQUENCE 701 AA; 79571 MW; 4733DBF018A4F3CD CRC64;

    MDLWNLSWFL FLDALLVISG LATPENFDVD GGMDQDIFDI NEGLGLDLFE

    GDIRLDRAQI RNSIIGEKYR WPHTIPYVLE DSLEMNAKGV ILNAFERYRL

    KTCIDFKPWA GETNYISVFK GSGCWSSVGN RRVGKQELSI GANCDRIATV

    QHEFLHALGF WHEQSRSDRD DYVRIMWDRI LSGREHNFNT YSDDISDSLN

    VPYDYTSVMH YSKTAFQNGT EPTIVTRISD FEDVIGQRMD FSDSDLLKLN

    QLYNCSSSLS FMDSCSFELE NVCGMIQSSG DNADWQRVSQ VPRGPESDHS

    NMGQCQGSGF FMHFDSSSVN VGATAVLESR TLYPKRGFQC LQFYLYNSGS

    ESDQLNIYIR EYSADNVDGN LTLVEEIKEI PTGSWQLYHV TLKVTKKFRV

    VFEGRKGSGA SLGGLSIDDI NLSETRCPHH IWHIRNFTQF IGSPNGTLYS

    PPFYSSKGYA FQIYLNLAHV TNAGIYFHLI SGANDDQLQW PCPWQQATMT

    LLDQNPDIRQ RMSNQRSITT DPFMTTDNGN YFWDRPSKVG TVALFSNGTQ

    FRRGGGYGTS AFITHERLKS RDFIKGDDVY ILLTVEDISH LNSTQIQLTP

    APSVQDLCSK TTCKNDGVCT VRDGKAECRC QSGEDWWYMG ERCEKRGSTR

    DTIVIAVSST VAVFALMLII TLVSVYCTRK KYRERMSSNR PNLTPQNQHA

    F

  • UCSC uc002kxj.5; human.;



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