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Human MTX3 ELISA Kit

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  • Alternative name

    Human metaxin 3 ELISA Kit;Human metaxin-3 ELISA Kit;
  • Catalog
    E024317
  • species
    Human
  • GeneMTX3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MMP-3. No significant cross-reactivity or interference between Human MMP-3 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman MTX3 ELISA Kit allows for the in vitro quantitative determination of MTX3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human MMP-3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human MMP-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of human MMP-3can be calculated.
  • Human Metaxin-3 Protein information
  • Uniprot ID MTX3_HUMAN
  • Uniprot AC Q5HYI7; B4DL65; E9PB57; Q7Z380; Q8NB92;
  • UniGene Hs.531418;
  • GeneID 345778
  • KEGG hsa:345778;
  • Human Metaxin-3 Protein SEQUENCE

  • SEQUENCE 312 AA; 35093 MW; 3B9BAB7CF64FF8EF CRC64;

    MAAPLELSCW GGGWGLPSVH SESLVVMAYA KFSGAPLKVN VIDNTWRGSR

    GDVPILTTED DMVSQPAKIL NFLRKQKYNA DYELSAKQGA DTLAYIALLE

    EKLLPAVLHT FWVESDNYFT VTKPWFASQI PFPLSLILPG RMSKGALNRI

    LLTRGQPPLY HLREVEAQIY RDAKECLNLL SNRLGTSQFF FGDTPSTLDA

    YVFGFLAPLY KVRFPKVQLQ EHLKQLSNLC RFCDDILSSY FRLSLGGISP

    AGQETVDANL QKLTQLVNKE SNLIEKMDDN LRQSPQLPPR KLPTLKLTPA

    EEENNSFQRL SP

  • UCSC uc003kge.5; human. [Q5HYI7-1];



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