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Human MAT2A ELISA Kit

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  • Alternative name

    Human adoMet synthase 2 ELISA Kit;Human methionine adenosyltransferase 2 ELISA Kit;Human MAT 2 ELISA Kit;Human Methionine adenosyltransferase II ELISA Kit;Human MAT-II ELISA Kit;Human AMS2 ELISA Kit;Human MATA2 ELISA Kit;Human MATII ELISA Kit;Human SAMS2 ELISA Kit;Human methionine adenosyltransferase 2A ELISA Kit;Human S-adenosylmethionine synthase isoform type-2 ELISA Kit;Human adoMet synthetase 2 ELISA Kit;Human methionine adenosyltransferase II, alpha ELISA Kit;Human testicular tissue protein Li 121 ELISA Kit;
  • Catalog
    E024333
  • species
    Human
  • GeneMAT2A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Methionine Adenosyltransferase II Alpha (MAT2a). No significant cross-reactivity or interference between Methionine Adenosyltransferase II Alpha (MAT2a) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman MAT2A ELISA Kit allows for the in vitro quantitative determination of MAT2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase II Alpha (MAT2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine Adenosyltransferase II Alpha (MAT2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human S-adenosylmethionine synthase isoform type-2 Protein information
  • Uniprot ID METK2_HUMAN
  • Uniprot AC P31153; A8K511; B4DN45; D6W5L1; Q53SP5;
  • UniGene Hs.516157;
  • GeneID 4144
  • KEGG hsa:4144;
  • Human S-adenosylmethionine synthase isoform type-2 Protein SEQUENCE

  • SEQUENCE 395 AA; 43661 MW; 2E7D1B91CC4F7BDD CRC64;

    MNGQLNGFHE AFIEEGTFLF TSESVGEGHP DKICDQISDA VLDAHLQQDP

    DAKVACETVA KTGMILLAGE ITSRAAVDYQ KVVREAVKHI GYDDSSKGFD

    YKTCNVLVAL EQQSPDIAQG VHLDRNEEDI GAGDQGLMFG YATDETEECM

    PLTIVLAHKL NAKLAELRRN GTLPWLRPDS KTQVTVQYMQ DRGAVLPIRV

    HTIVISVQHD EEVCLDEMRD ALKEKVIKAV VPAKYLDEDT IYHLQPSGRF

    VIGGPQGDAG LTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW

    VAKSLVKGGL CRRVLVQVSY AIGVSHPLSI SIFHYGTSQK SERELLEIVK

    KNFDLRPGVI VRDLDLKKPI YQRTAAYGHF GRDSFPWEVP KKLKY

  • UCSC uc002spr.4; human. [P31153-1];



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