Human MTR ELISA Kit

Alternative name
Human 5-methyltetrahydrofolate--homocysteine methyltransferase ELISA Kit;Human vitamin-B12 dependent methionine synthase ELISA Kit;Human MS ELISA Kit;Human HMAG ELISA Kit;Human cblG ELISA Kit;Human 5-methyltetrahydrofolate-homocysteine methyltransferase ELISA Kit;Human methionine synthase ELISA Kit;Human 5-methyltetrahydrofolate-homocysteine methyltransferase 1 ELISA Kit;Human cobalamin-dependent methionine synthase ELISA Kit;
Catalog
E024341
species
Human
GeneMTR
SpecificityThis assay has high sensitivity and excellent specificity for detection of MTR . No significant cross-reactivity or interference between MTR and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.375ng/ml
Intended UseHuman MTR ELISA Kit allows for the in vitro quantitative determination of MTR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MTR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MTR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MTR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MTR can be calculated.
Human Methionine synthase Protein information
Uniprot ID METH_HUMAN
Uniprot AC Q99707; A1L4N8; A9Z1W4; B7ZLW7; B9EGF7; Q99713; Q99723;
UniGene Hs.498187;
GeneID 4548
KEGG hsa:4548;
Human Methionine synthase Protein SEQUENCE
SEQUENCE 1265 AA; 140527 MW; B04C26BCBE9A57C2 CRC64;
MSPALQDLSQ PEGLKKTLRD EINAILQKRI MVLDGGMGTM IQREKLNEEH
FRGQEFKDHA RPLKGNNDIL SITQPDVIYQ IHKEYLLAGA DIIETNTFSS
TSIAQADYGL EHLAYRMNMC SAGVARKAAE EVTLQTGIKR FVAGALGPTN
KTLSVSPSVE RPDYRNITFD ELVEAYQEQA KGLLDGGVDI LLIETIFDTA
NAKAALFALQ NLFEEKYAPR PIFISGTIVD KSGRTLSGQT GEGFVISVSH
GEPLCIGLNC ALGAAEMRPF IEIIGKCTTA YVLCYPNAGL PNTFGDYDET
PSMMAKHLKD FAMDGLVNIV GGCCGSTPDH IREIAEAVKN CKPRVPPATA
FEGHMLLSGL EPFRIGPYTN FVNIGERCNV AGSRKFAKLI MAGNYEEALC
VAKVQVEMGA QVLDVNMDDG MLDGPSAMTR FCNLIASEPD IAKVPLCIDS
SNFAVIEAGL KCCQGKCIVN SISLKEGEDD FLEKARKIKK YGAAMVVMAF
DEEGQATETD TKIRVCTRAY HLLVKKLGFN PNDIIFDPNI LTIGTGMEEH
NLYAINFIHA TKVIKETLPG ARISGGLSNL SFSFRGMEAI REAMHGVFLY
HAIKSGMDMG IVNAGNLPVY DDIHKELLQL CEDLIWNKDP EATEKLLRYA
QTQGTGGKKV IQTDEWRNGP VEERLEYALV KGIEKHIIED TEEARLNQKK
YPRPLNIIEG PLMNGMKIVG DLFGAGKMFL PQVIKSARVM KKAVGHLIPF
MEKEREETRV LNGTVEEEDP YQGTIVLATV KGDVHDIGKN IVGVVLGCNN
FRVIDLGVMT PCDKILKAAL DHKADIIGLS GLITPSLDEM IFVAKEMERL
AIRIPLLIGG ATTSKTHTAV KIAPRYSAPV IHVLDASKSV VVCSQLLDEN
LKDEYFEEIM EEYEDIRQDH YESLKERRYL PLSQARKSGF QMDWLSEPHP
VKPTFIGTQV FEDYDLQKLV DYIDWKPFFD VWQLRGKYPN RGFPKIFNDK
TVGGEARKVY DDAHNMLNTL ISQKKLRARG VVGFWPAQSI QDDIHLYAEA
AVPQAAEPIA TFYGLRQQAE KDSASTEPYY CLSDFIAPLH SGIRDYLGLF
AVACFGVEEL SKAYEDDGDD YSSIMVKALG DRLAEAFAEE LHERVRRELW
AYCGSEQLDV ADLRRLRYKG IRPAPGYPSQ PDHTEKLTMW RLADIEQSTG
IRLTESLAMA PASAVSGLYF SNLKSKYFAV GKISKDQVED YALRKNISVA
EVEKWLGPIL GYDTD
UCSC uc001hyi.5; human. [Q99707-1];

Category

Human MTR ELISA Kit