Human MTHFR ELISA Kit

Alternative name
Human methylenetetrahydrofolate reductase ELISA Kit;Human 5,10-methylenetetrahydrofolate reductase (NADPH) ELISA Kit;Human methylenetetrahydrofolate reductase (NAD(P)H) ELISA Kit;
Catalog
E024370
species
Human
GeneMTHFR
SpecificityThis assay has high sensitivity and excellent specificity for detection of Methylenetetrahydrofolate Reductase (MTHFR). No significant cross-reactivity or interference between Methylenetetrahydrofolate Reductase (MTHFR) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 27.9pg/mL.
Intended UseHuman MTHFR ELISA Kit allows for the in vitro quantitative determination of MTHFR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylenetetrahydrofolate Reductase (MTHFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylenetetrahydrofolate Reductase (MTHFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylenetetrahydrofolate Reductase (MTHFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methylenetetrahydrofolate Reductase (MTHFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Methylenetetrahydrofolate reductase Protein information
Uniprot ID MTHR_HUMAN
Uniprot AC P42898; B2R7A6; Q5SNW6; Q5SNW9; Q7Z6M6; Q8IU73; Q9UQR2;
UniGene Hs.214142; Hs.737916;
GeneID 4524
KEGG hsa:4524;
Human Methylenetetrahydrofolate reductase Protein SEQUENCE
SEQUENCE 656 AA; 74597 MW; F16E774833D054B8 CRC64;
MVNEARGNSS LNPCLEGSAS SGSESSKDSS RCSTPGLDPE RHERLREKMR
RRLESGDKWF SLEFFPPRTA EGAVNLISRF DRMAAGGPLY IDVTWHPAGD
PGSDKETSSM MIASTAVNYC GLETILHMTC CRQRLEEITG HLHKAKQLGL
KNIMALRGDP IGDQWEEEEG GFNYAVDLVK HIRSEFGDYF DICVAGYPKG
HPEAGSFEAD LKHLKEKVSA GADFIITQLF FEADTFFRFV KACTDMGITC
PIVPGIFPIQ GYHSLRQLVK LSKLEVPQEI KDVIEPIKDN DAAIRNYGIE
LAVSLCQELL ASGLVPGLHF YTLNREMATT EVLKRLGMWT EDPRRPLPWA
LSAHPKRREE DVRPIFWASR PKSYIYRTQE WDEFPNGRWG NSSSPAFGEL
KDYYLFYLKS KSPKEELLKM WGEELTSEES VFEVFVLYLS GEPNRNGHKV
TCLPWNDEPL AAETSLLKEE LLRVNRQGIL TINSQPNING KPSSDPIVGW
GPSGGYVFQK AYLEFFTSRE TAEALLQVLK KYELRVNYHL VNVKGENITN
APELQPNAVT WGIFPGREII QPTVVDPVSF MFWKDEAFAL WIERWGKLYE
EESPSRTIIQ YIHDNYFLVN LVDNDFPLDN CLWQVVEDTL ELLNRPTQNA
RETEAP
UCSC uc001atc.3; human. [P42898-1];

Category

Human MTHFR ELISA Kit

Human methylenetetrahydrofolate reductase ELISA Kit;Human 5,10-methylenetetrahydrofolate reductase (NADPH) ELISA Kit;Human methylenetetrahydrofolate reductase (NAD(P)H) ELISA Kit;