Human MAP4 ELISA Kit

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  • Alternative name

    Human MAP-4 ELISA Kit;Human microtubule associated protein 4 ELISA Kit;Human microtubule-associated protein 4 ELISA Kit;

  • Catalog
    E024490
  • species
    Human
  • GeneMAP4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MAP4. No significant cross-reactivity or interference between MAP4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human MAP4 is typically less than 0.101ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
  • Intended UseHuman MAP4 ELISA Kit allows for the in vitro quantitative determination of MAP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MAP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MAP4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Microtubule-associated protein 4 Protein information
  • Uniprot ID MAP4_HUMAN
  • Uniprot AC P27816; Q13082; Q59FT2; Q68D74; Q6ZUW9; Q86V26; Q96A76; Q96NS9;
  • UniGene Hs.517949;
  • GeneID 4134
  • KEGG hsa:4134;
  • Human Microtubule-associated protein 4 Protein SEQUENCE
  • SEQUENCE 1152 AA; 121005 MW; 061A69AC18593339 CRC64;

    MADLSLADAL TEPSPDIEGE IKRDFIATLE AEAFDDVVGE TVGKTDYIPL

    LDVDEKTGNS ESKKKPCSET SQIEDTPSSK PTLLANGGHG VEGSDTTGSP

    TEFLEEKMAY QEYPNSQNWP EDTNFCFQPE QVVDPIQTDP FKMYHDDDLA

    DLVFPSSATA DTSIFAGQND PLKDSYGMSP CNTAVVPQGW SVEALNSPHS

    ESFVSPEAVA EPPQPTAVPL ELAKEIEMAS EERPPAQALE IMMGLKTTDM

    APSKETEMAL AKDMALATKT EVALAKDMES PTKLDVTLAK DMQPSMESDM

    ALVKDMELPT EKEVALVKDV RWPTETDVSS AKNVVLPTET EVAPAKDVTL

    LKETERASPI KMDLAPSKDM GPPKENKKET ERASPIKMDL APSKDMGPPK

    ENKIVPAKDL VLLSEIEVAQ ANDIISSTEI SSAEKVALSS ETEVALARDM

    TLPPETNVIL TKDKALPLEA EVAPVKDMAQ LPETEIAPAK DVAPSTVKEV

    GLLKDMSPLS ETEMALGKDV TPPPETEVVL IKNVCLPPEM EVALTEDQVP

    ALKTEAPLAK DGVLTLANNV TPAKDVPPLS ETEATPVPIK DMEIAQTQKG

    ISEDSHLESL QDVGQSAAPT FMISPETVTG TGKKCSLPAE EDSVLEKLGE

    RKPCNSQPSE LSSETSGIAR PEEGRPVVSG TGNDITTPPN KELPPSPEKK

    TKPLATTQPA KTSTSKAKTQ PTSLPKQPAP TTIGGLNKKP MSLASGLVPA

    APPKRPAVAS ARPSILPSKD VKPKPIADAK APEKRASPSK PASAPASRSG

    SKSTQTVAKT TTAAAVASTG PSSRSPSTLL PKKPTAIKTE GKPAEVKKMT

    AKSVPADLSR PKSTSTSSMK KTTTLSGTAP AAGVVPSRVK ATPMPSRPST

    TPFIDKKPTS AKPSSTTPRL SRLATNTSAP DLKNVRSKVG STENIKHQPG

    GGRAKVEKKT EAAATTRKPE SNAVTKTAGP IASAQKQPAG KVQIVSKKVS

    YSHIQSKCGS KDNIKHVPGG GNVQIQNKKV DISKVSSKCG SKANIKHKPG

    GGDVKIESQK LNFKEKAQAK VGSLDNVGHL PAGGAVKTEG GGSEAPLCPG

    PPAGEEPAIS EAAPEAGAPT SASGLNGHPT LSGGGDQREA QTLDSQIQET

    SI

  • UCSC uc003csb.3; human. [P27816-1];



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