Human MCM3 ELISA Kit

Alternative name
Human DNA polymerase alpha holoenzyme-associated protein P1 ELISA Kit;Human P1-MCM3 ELISA Kit;Human RLF subunit beta ELISA Kit;Human p102 ELISA Kit;Human HCC5 ELISA Kit;Human P1.h ELISA Kit;Human RLFB ELISA Kit;Human minichromosome maintenance complex component 3 ELISA Kit;Human DNA replication licensing factor MCM3 ELISA Kit;Human DNA replication factor MCM3 ELISA Kit;Human MCM3 minichromosome maintenance deficient 3 ELISA Kit;Human cervical cancer proto-oncogene 5 ELISA Kit;Human hRlf beta subunit ELISA Kit;Human minichromosome maintenance deficient 3 ELISA Kit;Human replication licensing factor, beta subunit ELISA Kit;
Catalog
E024581
species
Human
GeneMCM3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 3 (MCM3). No significant cross-reactivity or interference between Minichromosome Maintenance Deficient 3 (MCM3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman MCM3 ELISA Kit allows for the in vitro quantitative determination of MCM3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 3 (MCM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 3 (MCM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 3 (MCM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Minichromosome Maintenance Deficient 3 (MCM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human DNA replication licensing factor MCM3 Protein information
Uniprot ID MCM3_HUMAN
Uniprot AC P25205; B4DWW4; Q92660; Q9BTR3; Q9NUE7;
UniGene Hs.179565;
GeneID 4172
KEGG hsa:4172;
Human DNA replication licensing factor MCM3 Protein SEQUENCE
SEQUENCE 808 AA; 90981 MW; C967C068B7090558 CRC64;
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN
VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYV
GLEGSFGSKH VSPRTLTSCF LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA
TKKTIERRYS DLTTLVAFPS SSVYPTKDEE NNPLETEYGL SVYKDHQTIT
IQEMPEKAPA GQLPRSVDVI LDDDLVDKAK PGDRVQVVGT YRCLPGKKGG
YTSGTFRTVL IACNVKQMSK DAQPSFSAED IAKIKKFSKT RSKDIFDQLA
KSLAPSIHGH DYVKKAILCL LLGGVERDLE NGSHIRGDIN ILLIGDPSVA
KSQLLRYVLC TAPRAIPTTG RGSSGVGLTA AVTTDQETGE RRLEAGAMVL
ADRGVVCIDE FDKMSDMDRT AIHEVMEQGR VTIAKAGIHA RLNARCSVLA
AANPVYGRYD QYKTPMENIG LQDSLLSRFD LLFIMLDQMD PEQDREISDH
VLRMHRYRAP GEQDGDAMPL GSAVDILATD DPNFSQEDQQ DTQIYEKHDN
LLHGTKKKKE KMVSAAFMKK YIHVAKIIKP VLTQESATYI AEEYSRLRSQ
DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ
YAYFKKVLEK EKKRKKRSED ESETEDEEEK SQEDQEQKRK RRKTRQPDAK
DGDSYDPYDF SDTEEEMPQV HTPKTADSQE TKESQKVELS ESRLKAFKVA
LLDVFREAHA QSIGMNRLTE SINRDSEEPF SSVEIQAALS KMQDDNQVMV
SEGIIFLI
UCSC uc003pan.2; human. [P25205-1];

Category

Human MCM3 ELISA Kit