SpecificityThis assay has high sensitivity and excellent specificity for detection of human MT-ND6. No significant cross-reactivity or interference between human MT-ND6 and analogues was observed.
SensitivityThe minimum detectable dose of human MT-ND6 is typically less than 5.86 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman MT-ND6 ELISA Kit allows for the in vitro quantitative determination of MT-ND6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MT-ND6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MT-ND6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human NADH-ubiquinone oxidoreductase chain 6 Protein information
Uniprot ID
NU6M_HUMAN
Uniprot AC
P03923; Q34774; Q8HG30;
GeneID
4541
KEGG
hsa:4541;
Human NADH-ubiquinone oxidoreductase chain 6 Protein SEQUENCE